We narrowed to 144,768 results for: ins

1. pTriEx-mCherry-LOV2

   Plasmid

   #81041

   Purpose

   Mammalian or insect expression for wt LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV2
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV, p10

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDM1038

   Plasmid

   #108988

   Purpose

   Inducible expression vector (dox-on)

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Dictyostelium discoideum inducible expression vec…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEff

   Plasmid

   #97423

   Purpose

   Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

   Depositor

   Nikolai Ravin

   Article

   Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p40PX-EGFP

   Plasmid

   #19010

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p40phox (NCF4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-148 (PX domains)

   Promoter

   CMV

   Available since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcNDA-NES-PhoCl2c-mCherry

   Plasmid

   #164037

   Purpose

   Optogenetic control of protein localization by PhoCl2c

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-PhoCl2c-mCherry
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lyn-tailed mCherry-SEpHluorin

   Plasmid

   #32002

   Depositor

   Sergio Grinstein

   Article

   Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

   Insert

   Membrane-targeting sequence of Lyn kinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM-mTagBFP2

   Plasmid

   #228615

   Purpose

   Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR4-myc-nuc (w556-1)

   Plasmid

   #20026

   Purpose

   Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Gateway entry vector

   Tags

   Myc and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJL1 ATF1

    Plasmid

    #106286

    Purpose

    Expresses ATF1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    ATF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYS28

    Plasmid

    #202085

    Purpose

    Parent vector for producing universal minicircle donors for HaloTag C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT Halo-T2A-HygR Minicircle Parent
    

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    None

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAGFP-N1

    Plasmid

    #11909

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Has Service

    Cloning Grade DNA

    Insert

    pPAGFP-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    These plasmids are essentially the same as the Cl…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPAGFP-C1

    Plasmid

    #11910

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Has Service

    Cloning Grade DNA

    Insert

    PAGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    These plasmids are essentially the same as the Cl…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSAM_Bt

    Plasmid

    #112497

    Purpose

    Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

    Depositor

    Jeffrey Gordon, Andrew Goodman

    Article

    Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

    Inserts

    INSeq-adapted mariner transposon
    Himar1C9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2(+)-zGrad

    Plasmid

    #119716

    Purpose

    Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

    Depositor

    Holger Knaut

    Article

    Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

    Insert

    zGrad
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTM1_NP_ZEBOV

    Plasmid

    #69121

    Purpose

    Encodes for Ebola Virus Zaire NP under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    NP ZEBOV (NP )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHelper_TetR/pTet-Tnpase (camR)

    Plasmid

    #213910

    Purpose

    ATc-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDV05.2_pEF1a-iRFP720_secreted

    Plasmid

    #200409

    Purpose

    Expresses a secreted version of iRFP720 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (secreted)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDD104 (Peft-3::Cre)

    Plasmid

    #47551

    Purpose

    Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Insert

    Cre Recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-SF2

    Plasmid

    #17990

    Depositor

    Tom Misteli

    Article

    Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains SF2 (aa1-210)

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. CMV-Dre-T2A-TagRFP

    Plasmid

    #167968

    Purpose

    Transient expression of Dre and TagRFP

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    Dre recombinase
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBabe-tetoff-mVenus-PICdc42

    Plasmid

    #91876

    Purpose

    PI(WT): Photo inhibitable (blue light sensitive)

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-Rab5CA (Q79L)

    Plasmid

    #35140

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Constitutively Active- Q79L

    Promoter

    CMV

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PKC alpha CAT

    Plasmid

    #21234

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-325

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYFP

    Plasmid

    #175592

    Purpose

    Tet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    An improved monomeric YFP. See doi: 10.1021/bi051…

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCI-neo Flag HAUSP

    Plasmid

    #16655

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

    Insert

    HAUSP (USP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETZZtevPP7CP(C68A,C73A)

    Plasmid

    #27548

    Depositor

    Kathleen Collins

    Article

    Hogg et al RNA. 2007 Jun . 13(6):868-80.

    Insert

    Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
    

    Use

    Tags

    TEV protease cleavage site, six histidine tag, an…

    Expression

    Bacterial

    Mutation

    changed two cysteine to alanine (C68A,C73A) to in…

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. scFv-GCN4-sfGFP-deadTET1CD

    Plasmid

    #184442

    Purpose

    Catalytically DEAD TET1CD with scFv against GCN4

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    Catalytically DEAD TET1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBad-PhoCl2c

    Plasmid

    #164035

    Purpose

    Improved PhoCl variant with enhanced dissociation efficiency

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2c
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MCP-EGFP-PiggyBac

    Plasmid

    #190003

    Purpose

    Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

    Depositor

    Tineke Lenstra

    Article

    Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

    Insert

    MCP-EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge