We narrowed to 15,050 results for: pET
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Plasmid#169135PurposeE. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertCoh7
Tags3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…ExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-191~307-mEGFP(A206K)-TwStrep
Plasmid#208626Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 191~307, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-299~398-mEGFP(A206K)-TwStrep
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-del787-838-GGSG_O
Plasmid#147085PurposeBacterial Expression of NcQde-2_506-938-del787-838DepositorInsertNcQde-2_506-938-del787-838
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-del787-838-GSG_O
Plasmid#147086PurposeBacterial Expression of NcQde-2_506-938-del787-838DepositorInsertNcQde-2_506-938-del787-838
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Mortierella verticillate (delta prenylation motif)
Plasmid#170788PurposeExpresses pET-15b(+) Mortierella verticillate (delta prenylation motif)DepositorInsertMortierella verticillate PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Toxoplasma gondii (delta prenylation motif)
Plasmid#170789PurposeExpresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)DepositorInsertToxoplasma gondii PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)
Plasmid#166422PurposeExpress pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)DepositorInsertPlasmodium falciparum PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)
Plasmid#166423PurposeExpress pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)DepositorInsertPythium oligandrum PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)
Plasmid#166424PurposeExpress pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)DepositorInsertReticulomyxa filosa PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)
Plasmid#166425PurposeExpress pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)DepositorInsertSphaeforma arctica PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
Plasmid#166415PurposeExpress pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)DepositorInsertAcytostelium subglobosum PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)
Plasmid#166416PurposeExpress pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)DepositorInsertcaenorhabditis elegans PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Danio rerio PRL (Δprenylation motif)
Plasmid#166417PurposeExpress pET-15b(+) Danio rerio PRL (Δprenylation motif)DepositorInsertDanio rerio PRL (ptp4a3a Zebrafish)
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
Plasmid#166418PurposeExpress pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)DepositorInsertDrosophila melanogaster PRL (Prl Fly)
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)
Plasmid#166419PurposeExpress pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)DepositorInsertGaldieria sulphuraria PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)
Plasmid#166420PurposeExpress pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)DepositorInsertMonosiga brevicollis PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis
Plasmid#175788PurposePlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis
Plasmid#175801PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184Q W330YPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR
Plasmid#157672PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertCohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
TagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialPromoterT7Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher
Plasmid#157674PurposeExpression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertSpyCatcher with ddFLN4, ELP, Histag and ybbR tag
TagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialPromoterT7Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS
Plasmid#153445PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS
Plasmid#153444PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-GST-TNRC6A-(935-984)-NES-mut
Plasmid#42051DepositorInsertTNRC6A (TNRC6A Human)
TagsGST and HisExpressionBacterialMutationcontain aa935-984, F951A, F955A, I958A, F960AAvailable SinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
M61 pPET28c-Xenopus beta catenin domains R10C
Plasmid#17202DepositorAvailable SinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
M60 pPET28a-Xenopus beta catenin domains NR7
Plasmid#17201DepositorAvailable SinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT
Plasmid#111072PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
M57 pPET28a-TEV-full-length human beta catenin
Plasmid#17198DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only