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pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

Plasmid

#214877

Purpose

Lentiviral vector encoding RfxCas13d targeting LPT guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crLPT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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B1 Integrin

Plasmid

#186137

Purpose

For expression of recombinant beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP and untranslated His6 after stop codon

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2–mPKCε-CAT-HA

Plasmid

#190483

Purpose

Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

Depositor

Diomedes Logothetis

Article

Gada et al J Biol Chem. 2022 May;298(5):101893. doi

Insert

mCherry-CRY2–mPKCε-CAT-HA (Prkce Synthetic, Mouse)

Tags

Fused to CRY2, HA, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;G320E)

Plasmid

#33254

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and glycine 32…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
OXSM_pLX307

Plasmid

#98357

Purpose

Lentiviral expression of OXSM

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

OXSM (OXSM Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDSHA-ARL1-Myc

Plasmid

#67353

Purpose

Mammalian expression of hArl1 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
mCerulean3-Integrin-Beta1-N-18

Plasmid

#55423

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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T7T3-PacI-elfn1_459to1199

Plasmid

#87671

Purpose

In situ probe for mouse Elfn1

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Elfn1 (Elfn1 Mouse)

Expression

Bacterial

Mutation

partial sequence, (459 to 1199bp off NM_175522.3)

Promoter

T7/T3

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-ER-E2F2-dRb

Plasmid

#62805

Purpose

expresses an inducible E2F2 mutant that lacks the Rb binding domain

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

E2F2 (E2F2 Human)

Use

Retroviral

Tags

HA-Er

Expression

Mammalian

Mutation

deletion of amino acids 302-437, including the Rb…

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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His10-B1 Integrin

Plasmid

#186139

Purpose

For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP + uncleavable His10

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2 p15 4xSBR2 Mut 3' box

Plasmid

#15708

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR2 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutant 3' box. The 3' box contains the …

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pT2-cryR;dll4CE2-P1Egfp

Plasmid

#90147

Purpose

contains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E2P1Egfp (dll4 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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D998-EGFR-ET1

Plasmid

#44186

Depositor

Timothy Springer

Article

Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

Insert

D998-EGFR-ET1 (EGFR Human)

Tags

3C cleavge site, protein C peptide tag, his6, SBP…

Expression

Mammalian

Mutation

EGFR amino acids 1−998

Promoter

CVM

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmNot1_J

Plasmid

#146709

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Expression

Insect

Mutation

Three non silent mutations N605S, K759M, G1999S a…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL1

Plasmid

#67316

Purpose

hArl1 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA6-E2F4CA-Myc

Plasmid

#80428

Purpose

Mammalian expression of constitutively active chick E2F4

Depositor

José María Frade

Article

Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

Insert

E2F transcription factor 4 (E2F4 Chicken)

Tags

Myc

Expression

Mammalian

Mutation

T261E, T263E

Promoter

CMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARL1-V5-His6

Plasmid

#67437

Purpose

Mammalian expression of hArl1 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

His and V5

Expression

Mammalian

Mutation

bp 231 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
YAF2_pLX307

Plasmid

#98383

Purpose

Lentiviral expression of YAF2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

YAF2 (YAF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL1

Plasmid

#67458

Purpose

Mammalian expression of hArl1 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Expression

Mammalian

Mutation

bp 231 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only