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pEx-deletion-erm

Plasmid

#172213

Purpose

For allelic exchange in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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HLA_B5101

Plasmid

#165011

Purpose

Expression of HLA-B*51:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-B*51:01 (HLA-B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC23A2

Plasmid

#132025

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC23A2 (SLC23A2 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC7A1

Plasmid

#131925

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A1 (SLC7A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-FRB:Akt2

Plasmid

#72899

Purpose

Recruitable Akt2 (LocaTOR2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Serine/threonine protein kinase Bβ (AKT2 Human)

Use

Lentiviral

Tags

FRB(T2098L)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCGN-S2P-WT

Plasmid

#32957

Purpose

For mammalian expression of human S2P with an HA tag

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

S2P (MBTPS2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-PCTK2

Plasmid

#23845

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PCTK2 (CDK17 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
Zebrafish ArcLight Q175

Plasmid

#53616

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Zebrafish ArcLight-Q175 (tpte Zebrafish, Aequorea victoria, Synthetic)

Expression

Mammalian

Mutation

Dr-VSD contains R153Q mutation and an amino acid …

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

Plasmid

#171437

Purpose

Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

c-Fos-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_FLVCR1

Plasmid

#131892

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

FLVCR1 (FLVCR1 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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InsRA-TagBFP

Plasmid

#79791

Purpose

Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Tags

TagBFP extracellular

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MAP3K15-TAP

Plasmid

#69728

Purpose

Express tandem tagged MAP3K15 in cells

Depositor

Daniel Liebler

Insert

Apoptosis signal-regulating kinase 3 (MAP3K15 Human)

Tags

HA-Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-DYRK4

Plasmid

#23825

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK4 (DYRK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STYK1

Plasmid

#23903

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STYK1 (STYK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKCE

Plasmid

#23551

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCE (PRKCE Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-IHPK1

Plasmid

#23558

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IHPK1 (IP6K1 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NADK

Plasmid

#23747

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NADK (NADK Human)

Use

Gateway Cloning

Mutation

2MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKalpha FL WT

Plasmid

#33327

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Tags

FLAG

Expression

Mammalian

Promoter

SV40

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pSICO-CPSF6-eGFP

Plasmid

#167585

Purpose

Expresses human CPSF6 fused to eGFP

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

CPSF6-eGFP (CPSF6 Human)

Use

Lentiviral

Tags

eGFP

Mutation

WT

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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