We narrowed to 17,127 results for: URE

1. AAV phSyn1(S)-FLEX-tdTomato-WPRE

   Plasmid

   #51505

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   phSyn1

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT-TODestFLAGHAhFMRPiso1

   Plasmid

   #48690

   Purpose

   Expresses hFMRP iso 1

   Depositor

   Thomas Tuschl

   Article

   Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

   Insert

   FlagHA FMR1 Iso 1 (FMR1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   May 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV phSyn1(S)-FLEX-EGFP-WPRE

   Plasmid

   #51504

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   phSyn1

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1_MeCP2(deltaNIC)

   Plasmid

   #110191

   Purpose

   Expression of mouse MeCP2 (deltaNIC) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta N and C termini. Intervening region replace…

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNAI-GAL4-CREB-S133A

   Plasmid

   #46770

   Purpose

   Expression of GAL4 fusion to CREB-S133A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)
   

   Use

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 133 to Alanine

   Promoter

   CMV

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNAI-GAL4-CREB-S142A

   Plasmid

   #46771

   Purpose

   Expression of GAL4 fusion to CREB-S142A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)
   

   Use

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 142 to Alanine

   Promoter

   CMV

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pelB-MBP-BB0323-ss

   Plasmid

   #137067

   Purpose

   E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66700.1
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature BB0323: lacks the BB0323 signal peptide

   Promoter

   T7lac

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR-blunt-TOPO-FNDC5

   Plasmid

   #35970

   Depositor

   Bruce Spiegelman

   Article

   Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

   Insert

   fibronectin type III domain containing 5 (Fndc5 Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pMSCVpuro-eGFP-mcGAS

   Plasmid

   #108675

   Purpose

   For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

   Insert

   eGFP-cGAS (Cgas Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGmCherry-gRNA

    Plasmid

    #87110

    Purpose

    gRNA cloning vector with CAGmCherry

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1+_FH-AGO2-5xA

    Plasmid

    #92007

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1+_FH-AGO2-5xE

    Plasmid

    #92008

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR-53BP1 1-1972 WT

    Plasmid

    #53446

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    siRNA resistant and mutations A128V, T720A, A1347…

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. shRNA Caspase 1 (pSicoR mCherry)

    Plasmid

    #53575

    Purpose

    shRNA against human pro-caspase 1

    Depositor

    Warner Greene

    Article

    Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

    Insert

    shRNA Caspase 1 (CASP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; EF1a for mCherry

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA6.2-YFP-CWT

    Plasmid

    #176689

    Purpose

    Fluorescent reporter for phagosomal escape of Staphylococcus aureus

    Depositor

    Martin Fraunholz

    Article

    Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

    Insert

    cell wall-targeting domain of Lysostaphin
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    deletion of protase domain of Lysostaphin

    Promoter

    CMV

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBKWH-p53

    Plasmid

    #133900

    Purpose

    Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRESh-GFPII-SM-Dlk1

    Plasmid

    #108932

    Purpose

    GFP-tagged vector expressing full length isoform of Dlk1

    Depositor

    Anne Ferguson-Smith

    Article

    Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

    Insert

    Dlk1 (Dlk1 Mouse)
    

    Use

    Tags

    GFPII

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

    Plasmid

    #60814

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP-NLS (SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. mouse Oct4-GFP GOF18 transgenic reporter

    Plasmid

    #60527

    Purpose

    BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    GFPpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Oct4

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. AT5G63930 X059_pECIA2

    Plasmid

    #115116

    Purpose

    Bait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G63930 (AT5G63930 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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