We narrowed to 5,414 results for: PEP;

1. pHH0103_UBA2_ThiF

   Plasmid

   #109787

   Purpose

   Protein expression and purification of UBA2_ThiF

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   UBA2_ThiF (UBA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_ARPC3_P21-Arc

   Plasmid

   #109793

   Purpose

   Protein expression and purification of ARPC3_P21-Arc

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ARPC3_P21-Arc (ARPC3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)

   Plasmid

   #155136

   Purpose

   Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   D243G/L244G/F245G/M247G/S248G/M249G/A250G

   Promoter

   tac

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBB0238-TEV-His12

   Plasmid

   #137038

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66635.2
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_ATP5

   Plasmid

   #109857

   Purpose

   Protein expression and purification of ATP5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ATP5 (ATP5J Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

    Plasmid

    #106462

    Purpose

    Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(N163,242Q)
    

    Use

    Tags

    PA

    Expression

    Bacterial and Yeast

    Mutation

    changed Asparagines at positions 163 and 242 to G…

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

    Plasmid

    #106466

    Purpose

    Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(S219A)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Changed Serine 219 to Alanine

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

    Plasmid

    #106467

    Purpose

    Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(H474A)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Changed Histidine 474 to Alanine

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

    Plasmid

    #106468

    Purpose

    Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(D415A)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Changed Aspartate 415 to Alanine

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. csd6_4-330/pET28b(+) N-tag

    Plasmid

    #71351

    Purpose

    csd6, construct:4-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

17. csd6_13-330/pET28b(+) N-tag

    Plasmid

    #71352

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

18. csd6_1-330/pET28b(+) N-tag

    Plasmid

    #71350

    Purpose

    csd6, construct:1-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

19. SL542

    Plasmid

    #49951

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-8 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. SL544

    Plasmid

    #49970

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-10 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only