We narrowed to 3,005 results for: EED

1. pKF-P14MMP2AG-T2APuroR-5h

   Plasmid

   #128256

   Purpose

   Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   rtTA-Advanced::P2A::EGFP::T2A::PuroR
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG179 BC072452 3'UTR mut

   Plasmid

   #12037

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   N12 3'UTR mut (FAM107B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Promoter

   Available since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

3. psiCHECK-2-Rhoc-m_3'UTR

   Plasmid

   #31885

   Depositor

   Robert Blelloch

   Article

   Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

   Insert

   Rhoc mutant 3'UTR (RHOC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   miR-302b, 372 7-mer seed match mutated from AGCAC…

   Promoter

   SV40

   Available since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pAG73 PFTK1 3'UTR mut

   Plasmid

   #12061

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   PFTK1 3'UTR mut (CDK14 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Promoter

   Available since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG221 MDFIC 3'UTR mut

   Plasmid

   #12051

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   N22 3'UTR mut (MDFIC Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Promoter

   Available since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCS2-MfnG-EcYRS-EGFP*

   Plasmid

   #191119

   Purpose

   A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUS-miRFP670-TRF1

   Plasmid

   #227378

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric FUS IDR, WT (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. iLID-miRFP670-TRF1

   Plasmid

   #227385

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric iLID (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCHECK-1-miR-376a1(5p)-WT

   Plasmid

   #215875

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-376a1(5p)-WT (MIR376A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psiCHECK-1-miR-376a1(3p)-WT

    Plasmid

    #215876

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-WT (MIR376A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiCHECK-1-miR-589(5p)-WT

    Plasmid

    #215877

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-WT (MIR589 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psiCHECK-1-miR-589(3p)-WT

    Plasmid

    #215878

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-WT (MIR589 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. psiCHECK-1-miR-376a2(3p)-WT

    Plasmid

    #215879

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-WT (MIR376A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psiCHECK-1-miR-99a-WT

    Plasmid

    #215880

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psiCHECK-1-miR-376a1(5p)-I-type

    Plasmid

    #215882

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psiCHECK-1-miR-22(3p)-I-type

    Plasmid

    #215886

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psiCHECK-1-miR-376a2(3p)-I-type

    Plasmid

    #215887

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK-1-miR-99a-I-type

    Plasmid

    #215888

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. opt.a_pSTC0-zeo

    Plasmid

    #202347

    Purpose

    Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    opt.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR2_IHF_Mut

    Plasmid

    #149396

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is mutated at key positions needed f…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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