We narrowed to 7,284 results for: Tet-on

1. pICE-EGFP-FLAG-Ku70siR-Mut6E

   Plasmid

   #46962

   Purpose

   For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Mut6E corresponding to K282E K287E T300E K331E K3…

   Promoter

   CMV Tet

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2Apuro-TRE-synPCB2.0

   Plasmid

   #139481

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
   

   Use

   Tol2 transposon donor vector

   Expression

   Mammalian

   Promoter

   Tet responsive element

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO myc Separase S1226A/ C2029A

   Plasmid

   #59823

   Purpose

   Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S1226A/ C2029A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

   Plasmid

   #65662

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   introduced HindIII site (final goal to exchange F…

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5 FRT TO myc p31Comet

   Plasmid

   #59833

   Purpose

   Allows the integration of myc p31Comet in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Westhorpe et al J Cell Sci. 2011 Nov 15;124(Pt 22):3905-

   Insert

   p31 (MAD2L1BP Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK3b_029

   Plasmid

   #123807

   Purpose

   Encodes Tet-On 3G transactivator protein as a Type 3b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   TET3G
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235318

   Purpose

   Lentiviral expression of mRuby2 with 3' microRNA only

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235268

   Purpose

   Lentiviral expression of ComMAND base gene regulating mRuby2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235317

   Purpose

   Lentiviral expression of mRuby2 with intronic microRNA only

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

    Plasmid

    #205604

    Purpose

    Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

    Depositor

    Tarun Kapoor

    Article

    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

    Insert

    γ-tubulinΔGTP-RNAi-resistant-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G2228A, C2231T, C2237T, G2240A, N229A

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. H6GB1tevP53(304-393)

    Plasmid

    #200313

    Purpose

    bacterial expression of p53 TET-CTD

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53(304-393) (TP53 Human)
    

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-nsp7

    Plasmid

    #201025

    Purpose

    Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV-tet-ON

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK3_011

    Plasmid

    #123726

    Purpose

    Encodes HA-tagged PIF6 fused to Tet Repressor protein as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TETR::PIF6::HA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. miR-722 plix4.03

    Plasmid

    #97140

    Purpose

    Lentiviral tet-inducible expression of miR-722and Dendra 2

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    miR-722 (mir722 Zebrafish)
    

    Use

    Lentiviral

    Tags

    dendra2

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

    Plasmid

    #65660

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed glutamic acid 97 to alanine, lysine 100 t…

    Promoter

    CMV, 2x Tet

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 FRT TO myc Separase delta 55

    Plasmid

    #59824

    Purpose

    Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLJR965

    Plasmid

    #115163

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLIX403_Capture1_APOBEC_HA_P2A_mRuby

    Plasmid

    #183901

    Purpose

    Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Capture1_APOBEC_HA_P2A_mRuby

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PLJR962

    Plasmid

    #115162

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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