We narrowed to 10,458 results for: T7

1. pLz92

   Plasmid

   #226317

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-CsoS2B
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-a(+)-6xHis-mBlueberry(-9)

   Plasmid

   #219416

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   mBlueberry2(-9)
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   K137E, K199E, R234E

   Promoter

   T7

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HisMS2_VLP_FluB

   Plasmid

   #175956

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza B NSP 1 protein [B/Colorado/06/2017]

   Depositor

   Paul Freemont

   Article

   Unruh et al medRxiv 2024.04.17.24305965

   Insert

   NEP gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pD(P+T)_T7N

   Plasmid

   #223855

   Purpose

   4G-cloning donor for bacterial promoter and terminator, N-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD(P+T)_T7C

   Plasmid

   #223856

   Purpose

   4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1-mCherry (FspAI) (JM65)

   Plasmid

   #206445

   Purpose

   Expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-CdRdhA

   Plasmid

   #217824

   Purpose

   Expresses N-His-CdRdhA in E. coli

   Depositor

   Pamela Silver

   Article

   Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

   Insert

   N-His-CdRhdA
   

   Use

   Tags

   His6x

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDB179-RdhC1

   Plasmid

   #217822

   Purpose

   Expresses HisSUMO-RdhC1 in E. coli

   Depositor

   Pamela Silver

   Article

   Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

   Insert

   RdhC1
   

   Use

   Unspecified

   Tags

   His10xSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDB179-RdhF3

   Plasmid

   #217823

   Purpose

   Expresses HisSUMO-RdhF3 in E. coli

   Depositor

   Pamela Silver

   Article

   Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

   Insert

   RdhF3
   

   Use

   Tags

   His10xSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-DhFld

    Plasmid

    #217821

    Purpose

    Expresses N-His-DhFld in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    DhFld
    

    Use

    Tags

    His6x

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-eIF4E-TwStrep

    Plasmid

    #208638

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

    Depositor

    Quanfu Ma

    Insert

    human eIF4E (ENST00000450253) (EIF4E Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-mEGFP(A206K)-TwStrep

    Plasmid

    #208622

    Purpose

    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone

    Depositor

    Quanfu Ma

    Insert

    mEGFP(A206K)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    (compared with pEGFP-C1, protein level) A206K

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid

    #164055

    Purpose

    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mouse KANK4 Ankyrin Repeats pET151

    Plasmid

    #164057

    Purpose

    Expresses mouse KANK4 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET51b_rHCaMP

    Plasmid

    #187106

    Purpose

    Expression of rHCaMP in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    rHCaMP
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJC20_cam1-T6C

    Plasmid

    #182427

    Purpose

    Bacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1 with the T6C residue replacement to allow labelling

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    cam1 (cam1 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T6C substitution

    Promoter

    T7

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT5T_Rev7_1-211_SA112_KVK

    Plasmid

    #105634

    Purpose

    Expresses mouse monomeric Rev7 protein

    Depositor

    Thanos Halazonetis

    Insert

    Rev7 (Mad2l2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAcrVIA4

    Plasmid

    #164863

    Purpose

    Inducible expression of AcrVIA4 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    AcrVIA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcrVIA7

    Plasmid

    #164864

    Purpose

    Inducible expression of AcrVIA7 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    AcrVIA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 38 pCOLI_K_ C_STREP

    Plasmid

    #160266

    Purpose

    C-term 2xStrepII uncleavable Kan resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge