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1352wUV2 F2bbs kan GCANNNAGGk

Plasmid

#18752

Depositor

Scot Wolfe

Publication

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pSL1749 (pDonor, R*MmeI)

Plasmid

#160733

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Publication

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA

Expression

Bacterial

Promoter

N/A

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p2lox-cAMPr R739A

Plasmid

#102618

Purpose

cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

Depositor

Justin Blau

Publication

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

R739A

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-E SCT-T2A-GFP

Plasmid

#205461

Purpose

lentiviral plasmid for expression of HLA-E SCT

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HLA-E SCT (HLA-E Human)

Expression

Mammalian

Promoter

UBC

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-mRFP-pcDNA3.1-

Plasmid

#52510

Purpose

expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Tags

FLAG-HA

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMOD_A1510

Plasmid

#91036

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

ZmUbi

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TRE-DIO-mRuby2

Plasmid

#99117

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-mTurquoise2

Plasmid

#99115

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-Gal4

Plasmid

#53366

Purpose

full-length native Gal4 with stop in Gateway middle entry clone

Depositor

Joshua Bonkowsky

Publication

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

native (full-length) Gal4

Promoter

no promoter

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-tTA

Plasmid

#175280

Purpose

AAV vector mediating neuron-specific expression of tTA

Depositor

Wei Xu

Publication

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

tTA advanced (improved tetracycline-controlled transactivator)

Use

AAV

Promoter

Synapsin

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pZ143-pcDNA3-BvCas12b

Plasmid

#122445

Purpose

Expresses BvCas12b in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CMV-NLS-BvCas12b-NLS-3xHA

Tags

3XHA

Expression

Mammalian

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47870

Purpose

All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

NmCas9
U6pr-tracrRNA
OCT4 crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-WT

Plasmid

#30370

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

wild type

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAAV-hsyn-flex-Positron-ST

Plasmid

#129267

Purpose

Cre-dependant expression of soma-localized Positron in neurons

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

AAV

Expression

Mammalian

Mutation

D92N, E199V

Promoter

hsyn

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA6213S

Plasmid

#122094

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Promoter

Pem7

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_HaloTag9

Plasmid

#175545

Purpose

Expression of HaloTag9 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag7

Plasmid

#169330

Purpose

Expression of TOMM20-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag7 (TOMM20 Human)

Tags

HaloTag7

Expression

Mammalian

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Caspase 12

Plasmid

#35574

Depositor

Junying Yuan

Publication

Nakagawa et al Nature. 2000 Jan 6. 403(6765):98-103.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caspase 12 (Casp12 Mouse)

Expression

Mammalian

Available since

May 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
L40C-CRISPR.EFS.mNeon

Plasmid

#69146

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Publication

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-SIN-GFP-PTEN-WT

Plasmid

#30391

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP-PTEN-WT (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-WT

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only