We narrowed to 14,607 results for: sta

Showing: 2881 - 2900 of 14607 results

Results per page:

Sort results by:

pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Expression

Bacterial

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Chicken Mermaid S188

Plasmid

#53617

Purpose

Genetically encoded voltage sensor based on mUKG-mKOk FRET pair

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken Mermaid S188

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN-D2irTNG4kwh

Plasmid

#44722

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
htetR::NLS::EGFP

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-JEDI-2P-Kv-WPRE

Plasmid

#179463

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P-Kv

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hSyn-PdCO-mScarlet-WPRE

Plasmid

#198510

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

Plasmid

#202199

Purpose

Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

stChrimsonR, PdCO

Use

AAV and Cre/Lox

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

Plasmid

#198516

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hSyn-PdCO-EGFP-WPRE

Plasmid

#198513

Purpose

Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJB38-NWMN2930

Plasmid

#84457

Purpose

pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

Staphylococcus aureus NWMN genomic region NWMN29-30

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPtGE35

Plasmid

#107999

Purpose

Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain

Use

CRISPR and Synthetic Biology; Episomal vector for…

Tags

Cas9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
pE-SUMO CRYAA

Plasmid

#80756

Purpose

Expression of human alpha A-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYAA (CRYAA Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
punc-119cR

Plasmid

#21596

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119:mCherry (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Tags

mCherry

Expression

Worm

Mutation

N/A

Available Since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE792

Plasmid

#167300

Purpose

Vector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIRECT_26H

Plasmid

#91150

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

Plasmid

#20293

Purpose

Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Alanine

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE EF1α dCas9-NED

Plasmid

#109369

Purpose

Lentiviral vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

EF-1α

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

Plasmid

#84603

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

ODC1 destabilizing element

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge