We narrowed to 21,787 results for: Tes

1. pSliceRep_B_miR-7.M4

   Plasmid

   #220469

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   SUMO_Eu1
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEARBOCS-v0- Gfap-TagIN-mCherry

   Plasmid

   #196490

   Purpose

   To tag endogenous mouse GFAP with mCherry

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Inserts

   mCherry
   gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLX-EF1a_NKX2-5

   Plasmid

   #221491

   Purpose

   NKX2-5 lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-5 (NKX2-5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223_NKX2-1

   Plasmid

   #221472

   Purpose

   Gateway cloning of NKX2-1 short isoform overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pReporter_mNeonGreen

   Plasmid

   #213838

   Purpose

   Facilitates genomic integration of the control reporter without zinc finger binding sites.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

   Plasmid

   #208628

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 1~41, other residues not included

   Promoter

   T7

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUdO4c

   Plasmid

   #210233

   Purpose

   low-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. B2401_miRFP-FKBP5x

   Plasmid

   #215118

   Purpose

   Expresses miRFP-FKBP-FKBP-FKBP-FKBP-FKBP in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   miRFP-FKBP-FKBP-FKBP-FKBP-FKBP
   

   Use

   Tags

   miRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUdO4a

   Plasmid

   #210229

   Purpose

   low-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUdO2a

    Plasmid

    #210227

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

    Plasmid

    #208630

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    Δ1~34,Δ215~284

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

    Plasmid

    #208632

    Purpose

    for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 53~77, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

    Plasmid

    #208624

    Purpose

    for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 1~77, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1-Dm7SL-RNA-long_M

    Plasmid

    #146891

    Purpose

    Insect Expression of Dm7SL-RNA-long

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    Dm7SL-RNA-long (7SLRNA:CR32864 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1-Dm7SL-RNA-short_M

    Plasmid

    #146892

    Purpose

    Insect Expression of Dm7SL-RNA-short

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    Dm7SL-RNA-short (7SLRNA:CR32864 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmCup_1-417_M

    Plasmid

    #146895

    Purpose

    Insect Expression of DmCup_1-417

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_1-417 (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmCup_1-779_M

    Plasmid

    #146902

    Purpose

    Insect Expression of DmCup_1-779

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_1-779 (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-DmCup_418-1132_M

    Plasmid

    #146906

    Purpose

    Insect Expression of DmCup_418-1132

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_418-1132 (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmCup-L379AL383A_M

    Plasmid

    #146912

    Purpose

    Insect Expression of DmCup-L379AL383A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup-L379AL383A (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-CS-DmCup_311-440_M

    Plasmid

    #146913

    Purpose

    Bacterial Expression of DmCup_311-440

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_311-440 (cup Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EGFP-ShadowY

    Plasmid

    #193555

    Purpose

    Expresses mEGFP-ShadowY in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    ShadowY
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LentiCRISPR v2 sgZER1-1

    Plasmid

    #191549

    Purpose

    Expression of spCas9 and sgRNA targetting ZER1

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    spCas9 (ZER1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LentiCRISPR v2 sgZER1-2

    Plasmid

    #191550

    Purpose

    Expression of spCas9 and sgRNA targetting ZER1

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    spCas9 (ZER1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SpAGS-dGoLoco1-GFP

    Plasmid

    #185577

    Purpose

    for overexpression

    Depositor

    Mamiko Yajima

    Article

    Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

    Insert

    AGS-dGoLoco1
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    GFP

    Expression

    Mutation

    deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYTK001_P2Aq(2nd)

    Plasmid

    #183089

    Purpose

    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    P2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC-TalO8 (Blank-CoTrIP)

    Plasmid

    #178303

    Purpose

    Blank CoTrIP plasmid

    Depositor

    Brian Zid

    Article

    Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

    Type

    Empty backbone

    Use

    Tags

    NA

    Expression

    Yeast

    Mutation

    Promoter

    NA

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NK88

    Plasmid

    #176612

    Purpose

    To manipulate ligand spacing.

    Depositor

    Meghan Morrissey

    Article

    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

    Insert

    DNA-CARy- aTM (aa291-404)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-EGFP-DmCup_771-1132_C

    Plasmid

    #146030

    Purpose

    Insect Expression of DmCup_771-1132

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_771-1132 (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-lambdaN-HA-DmCup_771-1132_C

    Plasmid

    #146034

    Purpose

    Insect Expression of DmCup_771-1132

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_771-1132 (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-lambdaN-HA-DmCup-Y342AL347A_C

    Plasmid

    #146035

    Purpose

    Insect Expression of DmCup-Y342AL347A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup-Y342AL347A (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge