We narrowed to 6,926 results for: c myc vectors

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p944-SWITCH-OVER insert: EF1a-Puro

Plasmid

#217891

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

Plasmid

#52539

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNA500

Plasmid

#149583

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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HM3C NaVAe1_p pET24b

Plasmid

#127854

Purpose

Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1p

Tags

3C Cleavage site, His, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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mcherry-p62 Double Mutation/Silent

Plasmid

#187982

Purpose

K7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D7A, D69A, silent mutation

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPA-RL-FL

Plasmid

#78863

Purpose

DULIP Tandem Construct used for data normalization

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

Firefly

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pQnl_tet

Plasmid

#191355

Purpose

Clostridium expression vector with TetR-repressed NanoLuc expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette

Expression

Bacterial

Promoter

PGus2A-TetO2/1

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4_FLAG/HA_FUS_His6

Plasmid

#26365

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FUS (FUS Human)

Use

Entry vector

Tags

FLAG/HA and His6

Mutation

delta 527 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

Plasmid

#149588

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC12)_Psyn-sgRNArodA

Plasmid

#149661

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC12), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

pSUP35, min pCYC1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

Plasmid

#149590

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

Plasmid

#149594

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1 Insulator ObLiGaRe/EPB71

Plasmid

#90018

Purpose

Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

Depositor

Eric-Paul Bennett

Article

Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

Insert

MCS flanked by insulator and inverted ZFN binding sites

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52537

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52538

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb

Plasmid

#109579

Purpose

Multisite gateway vector for 3' tagging with RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNArodA

Plasmid

#149656

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Dnm2b-DN

Plasmid

#109574

Purpose

Multisite gateway vector for 3' tagging with dominant negative Dynamin2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2b (dnm2b Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR4_EWSR1_G2A_R650H

Plasmid

#26372

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Mutation

R650H

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
pPU-HSA-miR30-L1221

Plasmid

#174221

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-HSA-miR30-MDA5

Plasmid

#174222

Purpose

All-in-one control rescue-MDA5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pQmod3S-GG

Plasmid

#191349

Purpose

Clostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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ppih.001.177.W133A

Plasmid

#137655

Purpose

Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

ppih (PPIH Human)

Tags

GST-(His)6-thrombin

Expression

Bacterial

Mutation

001-177-W133A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E3E4 PUb-YFP

Plasmid

#173666

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
YFP

Tags

SV40 NLS

Expression

Insect

Mutation

Nix isoforms with exons E1, E3, E4

Promoter

Aedes aegypti PUb and own

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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CPL2g/pDONR221

Plasmid

#172093

Purpose

Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Use

Gateway entry vector

Promoter

CPL2

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 CARD8 FIIND

Plasmid

#169963

Purpose

pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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