We narrowed to 16,109 results for: form

1. KRAS2B

   Plasmid

   #55655

   Purpose

   expression of mouse KRAS2B

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   KRAS2B (Kras Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pBeast-pAhpC-RBS- sfGFP

   Plasmid

   #190051

   Purpose

   GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3 Flag MKK4beta

   Plasmid

   #15517

   Depositor

   Roger Davis

   Article

   Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

   Insert

   pCDNA3 Flag MKK4beta (MAP2K4 Human)
   

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

4. tagSrc(WT)-mCer3 S208F/V224L

   Plasmid

   #179138

   Purpose

   mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   Src, SsrA
   

   Tags

   mCerulean3 (S208F/V224L)

   Expression

   Mammalian

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. R26TV LSL-TRL

   Plasmid

   #68476

   Purpose

   Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Cre/Lox, Luciferase, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 LIC 6A CARD8-FLAG S297A

   Plasmid

   #169949

   Purpose

   Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S297A

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLXSN-H-Ras_V12

   Plasmid

   #39516

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS V12 (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G12V

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-mCherry-TSC2(AA)

   Plasmid

   #192442

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A) (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetro-XT-PAK2-CFP(puro)-L107F

   Plasmid

   #81180

   Purpose

   Retroviral vector for expression of kinase active PAK2 with a CFP fusion

   Depositor

   Angeliki Malliri

   Article

   Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

   Insert

   p21 (RAC1) activated kinase 2 (PAK2 Human)
   

   Use

   Retroviral

   Tags

   CFP

   Mutation

   L107F

   Promoter

   P-tight TRE

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

10. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP779_αGCN4-VP64-CO

    Plasmid

    #211772

    Purpose

    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-VP64
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect VP64 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV tubulin myc

    Plasmid

    #12581

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    tubulin (TUBG1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    *

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSVE Grb2 myc 49L/203R

    Plasmid

    #26087

    Depositor

    Bob Weinberg

    Article

    Egan et al Nature. 1993 May 6. 363(6424):45-51.

    Insert

    Grb2 49L/203R (GRB2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    49L/203R

    Available Since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

14. Sema6d.1-AP-His

    Plasmid

    #72042

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sema6d.1-Fc-His

    Plasmid

    #72168

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. KK501: pMVP (L3-L2) FKBP DD + polyA

    Plasmid

    #121789

    Purpose

    pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FKBP Degron Domain + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBXNPHM3-Nb_MsbA#1

    Plasmid

    #186428

    Purpose

    Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1

    Depositor

    Markus Seeger

    Article

    Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi

    Insert

    Nanobody Nb_MsbA#1
    

    Use

    Affinity Reagent/ Antibody

    Tags

    3C cleavage site, His Tag, Maltose Binding Protei…

    Promoter

    pBAD

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUW-TetO-SP110

    Plasmid

    #178448

    Purpose

    doxycycline-inducible expression of Human SP110 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    SP110 (SP110 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223 LC3A G120A

    Plasmid

    #123070

    Purpose

    Gateway entry clone encoding human MAP1LC3A G120A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 120 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. B2387_mEGFP-FKBP2x

    Plasmid

    #216216

    Purpose

    Expresses mEGFP(A206K) fused to FKBP-FKBP in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    mEGFP-FKBP-FKBP (FKBP1A Human)
    

    Tags

    EGFP(A206K)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IL2 F42A pAcGP67a

    Plasmid

    #41807

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Tags

    6x His

    Expression

    Insect

    Mutation

    Phenylalanine 42 to Alanine

    Promoter

    Polyhedron

    Available Since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

22. LLP790_αGCN4-EZH2-FL-CO

    Plasmid

    #211784

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-KRAB
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LLP789_αGCN4-DNMT3A-CO

    Plasmid

    #211783

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26570

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pWZL Blast H-Ras G12V T35S

    Plasmid

    #12278

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V T35S (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The T35S mutat…

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pSLIK-Venus-TmiR-Luc

    Plasmid

    #25739

    Purpose

    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUW-TetO-SUB1

    Plasmid

    #178440

    Purpose

    doxycycline-inducible expression of Human SUB1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    SUB1 (SUB1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Arabidopsis thaliana Atm3 d80 pET21a+

    Plasmid

    #173045

    Purpose

    Expresses AtAtm3 in E.coli

    Depositor

    Douglas Rees

    Article

    Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

    Insert

    Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    with N-terminal 80 amino acids deletion

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRL K-ras 3'UTR mutant

    Plasmid

    #14805

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    K-ras 3'UTR (Kras Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    let-7 miRNA binding site mutations

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

30. Bin1 N-BAR domain

    Plasmid

    #168280

    Purpose

    Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector

    Depositor

    Cheng-han Yu

    Article

    Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

    Insert

    Bin1 (Bin1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-267

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 Hmga2 tr

    Plasmid

    #14793

    Depositor

    David Bartel

    Article

    Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

    Insert

    Hmga2 truncated (Hmga2 Mouse)
    

    Expression

    Mammalian

    Mutation

    truncataed HMGA2. As in human tumors, Hmga2-tr la…

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108849

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    nMag

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMGIB-HA-hTCAB1

    Plasmid

    #167460

    Purpose

    Retroviral expression of HA-TCAB1 (WDR79) in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

    Insert

    TCAB1 (WRAP53 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR223 GABARAP G116A

    Plasmid

    #123081

    Purpose

    Gateway entry clone encoding human GABARAP G116A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 116 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

    Plasmid

    #121835

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMRX-IPU-FLAG-NEK9T210A

    Plasmid

    #168270

    Purpose

    Expresses kinase-dead NEK9 mutant in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 210 to alanine

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223 LC3B G120A

    Plasmid

    #123073

    Purpose

    Gateway entry clone encoding human MAP1LC3B G120A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 120 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFPm-DAD M1041A

    Plasmid

    #25411

    Depositor

    Arthur Alberts

    Article

    Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

    Insert

    Diap3 (Diaph3 Mouse)
    

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Alanine substitution at critical residue in DAD t…

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pHisAlix deltaPRD

    Plasmid

    #42577

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
    

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

42. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

    Plasmid

    #121824

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/LSD1 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. RCAS(A) dRhoA V14 (CT#846)

    Plasmid

    #13949

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    RhoA (Rho1 Fly)
    

    Use

    Retroviral; Avian expression

    Mutation

    G14V mutation

    Available Since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

44. pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

    Plasmid

    #89870

    Purpose

    expresses DRD2-iTango component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSG5-Myr-FLAG-p110α-DM

    Plasmid

    #55713

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    T207D, K227A

    Promoter

    T7

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

46. pcDNA3 merlin 1-332

    Plasmid

    #11625

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino-terminal half of merlin.

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

47. pCDF1-p18INK4c

    Plasmid

    #25650

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Apr 15. 68(8):2564-9.

    Insert

    p18INK4c (CDKN2C Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

48. pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

    Plasmid

    #164011

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75177

    Purpose

    Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Three prime entry vector for multisite gateway th…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Sema4f.b-Fc-His

    Plasmid

    #72159

    Purpose

    Expresses the extracellular region of the Sema4F, isoform b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4f.b (Sema4f Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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