Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 2,966 results for: ER;

Showing: 2921 - 2940 of 2966 results

  1. pPBbsr2-EREG-ScNeo

    Plasmid
    #209909
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EREG-ScNeo (EREG Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209909-plasmid-map-sequence-id-416692

    Enlarge

  2. pPBbleo-TGFa-EREG-ScNeo

    Plasmid
    #209915
    Purpose
    To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    TGFa-EREG-ScNeo (EREG, TGFA Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209915-plasmid-map-sequence-id-416705

    Enlarge

  3. pPBbleo-EREG-ScNeo

    Plasmid
    #209905
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EREG-ScNeo (EREG Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209905-plasmid-map-sequence-id-416688

    Enlarge

  4. pAW-BiP-Nb127D01-mCherry-KDEL

    Plasmid
    #171575
    Purpose
    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane
    Depositor
    Norbert Perrimon, Hidde Ploegh
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
    Use
    Tags
    BiP signal peptide and mCherry-KDEL
    Expression
    Insect
    Mutation
    Promoter
    fly actin5C promoter
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    171575-plasmid-map-sequence-id-339963

    Enlarge

  5. pAW-BiP-NbVHH05-mCherry-KDEL

    Plasmid
    #171574
    Purpose
    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane
    Depositor
    Norbert Perrimon, Hidde Ploegh
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
    Use
    Tags
    BiP signal peptide and mCherry-KDEL
    Expression
    Insect
    Mutation
    Promoter
    fly actin5C promoter
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171574-plasmid-map-sequence-id-336399

    Enlarge

  6. TFORF3132

    Plasmid
    #144608
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144608-plasmid-map-sequence-id-346764

    Enlarge

  8. TFORF3305

    Plasmid
    #144781
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR1 (ESR1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144781-plasmid-map-sequence-id-404373

    Enlarge

  9. TFORF1954

    Plasmid
    #142296
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142296-plasmid-map-sequence-id-395774

    Enlarge

  10. TFORF1955

    Plasmid
    #142297
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142297-plasmid-map-sequence-id-454963

    Enlarge

  11. TFORF1956

    Plasmid
    #142898
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142898-plasmid-map-sequence-id-396065

    Enlarge

  12. TFORF1957

    Plasmid
    #142298
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142298-plasmid-map-sequence-id-395775

    Enlarge

  13. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid
    #73209
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73209-plasmid-map-sequence-id-139734

    Enlarge

  19. str-KDEL_Stx5L(M55V)-SBP-mCitrine

    Plasmid
    #209846
    Purpose
    Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    STX5 (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209846-plasmid-map-sequence-id-413647

    Enlarge

  20. str-KDEL_Stx5S-SBP-mCitrine

    Plasmid
    #154845
    Purpose
    Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Short isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Lacks amino acids 1-54, enocdes short isoform of …
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154845-plasmid-map-sequence-id-325019

    Enlarge

Showing: 2921 - 2940 of 2966 results