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pAW8ENdeI-spo5DSR

Plasmid

#48983

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pIBG-FTet

Plasmid

#33361

Depositor

Doug Mortlock

Article

Chandler et al Mol Cell Biol. 2007 Apr;27(8):2934-51. E

Inserts

IRES-Bgeo
TetR cassette

Use

Mouse Targeting

Tags

FRT site

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pBY2807

Plasmid

#23222

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

Gateway-SnAvi

Expression

Bacterial, Insect, and Mamm…

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
pFC663

Plasmid

#27324

Depositor

Fred Cross

Article

Cross et al Genetics. 2007 Jul . 176(3):1541-55.

Insert

SIC1 (SIC1 Budding Yeast)

Expression

Yeast

Mutation

Contains insertion relative to SGDB sequence of G…

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-8XDSR

Plasmid

#48981

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-3XDSR

Plasmid

#48976

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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PQCXIP_mcherry_ALPHA1-782_ERTAG

Plasmid

#252254

Purpose

Express LacZ alpha 1-782-ERtag fusion

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

LACZ

Use

Retroviral

Mutation

LACZ 1-782

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pJPC060

Plasmid

#234535

Purpose

TRV2 expressing FLPe

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

FLPe recombinase

Use

Recombinase

Expression

Plant

Promoter

CaMV 35S

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ6-HIS5

Plasmid

#228259

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

his5

Expression

Yeast

Promoter

TEF1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmN-C47A

Plasmid

#221367

Purpose

Express C47A ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN-C47A

Tags

His6

Expression

Bacterial

Mutation

changed Cys 47 to Ala

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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Sp(AU)sgRNA-mKO2

Plasmid

#184443

Purpose

sgRNA with AU flip (sgRNA2.0), mKO2 as selection marker

Depositor

Rhys Allan

Article

Chan et al Immunol Cell Biol. 2020 Sep 24. doi: 10.

Insert

sgRNA with AU flip, mKO2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMV23

Plasmid

#128887

Purpose

Position 4 module- STOP (stop codon)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

STOP

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMV10

Plasmid

#128892

Purpose

Position 6 module- URA3

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

URA3 (URA3 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV5

Plasmid

#128886

Purpose

Position 4 module- CLN2-PEST (Degron)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

CLN2-PEST

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Rabbit NHERF-1

Plasmid

#31644

Depositor

Edward Weinman

Article

Weinman et al J Clin Invest. 1995 May . 95(5):2143-9.

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Expression

Bacterial

Mutation

none

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_PsChR2_EYFP

Plasmid

#69057

Purpose

High-efficiency blue-shifted cation channelrhodopsin

Depositor

John Spudich

Article

Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

Insert

PsChR2

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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LES2A-CRE

Plasmid

#167933

Purpose

Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

mScarlet-P2A-CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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