165,075 results

1. MYC-hTRAK2

   Plasmid

   #225142

   Purpose

   Expresses MYC-tagged human TRAK2

   Depositor

   Thomas Schwarz

   Article

   Davis et al Life Sci Alliance. 2022 Oct 27;6(1):e202

   Insert

   TRAK2 (TRAK2 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

   Plasmid

   #181792

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. scFv-sfGFP-DNMT3A1

   Plasmid

   #102278

   Purpose

   The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

   Depositor

   Margaret Goodell

   Article

   Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

   Insert

   DNMT3A1 (DNMT3A Human)
   

   Use

   Lentiviral

   Tags

   scFV-sfGFP

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

   Plasmid

   #125713

   Purpose

   Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV1 and AAV5

   Insert

   eOPN3
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   hSyn1

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGK-HALO-CTERM-GFP

   Plasmid

   #185777

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4-TO-Puromycin-mVenus-MAP

   Plasmid

   #44118

   Depositor

   Dannel McCollum

   Article

   Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

   Type

   Empty backbone

   Tags

   mVenus-MAP

   Expression

   Mammalian

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSicoR human Drosha1

   Plasmid

   #14766

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Drosha shRNA (DROSHA Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

8. Tag5Amyc-GSK3b KD

   Plasmid

   #16262

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Kinase dead: K85A

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. Eef1a-1955/-1>nls::Cas9::nls

   Plasmid

   #59987

   Purpose

   Eef1a (EF1alpha) promoter driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Eef1a (EF1alpha) -1955/-1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNCST-avGFP

    Plasmid

    #129508

    Purpose

    Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    avGFP
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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