We narrowed to 14,439 results for: eng
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Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialPromoterT7Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Endoplasmic reticulum-targeted optoPLD
Plasmid#140060PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPhospholipase D
TagsCIBN, CRY2, P2A, Sac1(TM), and mCherryExpressionMammalianAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/TO-hEPO
Plasmid#50437Purposeexpress human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery systemDepositorInsertEPO (EPO Human)
UseLentiviral; Tetracycline-regulatable (t-rex)ExpressionMammalianPromoterCMV/TOAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRL-MHCK7-R-GECO
Plasmid#109374PurposeExpresses calcium indicator R-GECO in muscle cellsDepositorInsertR-GECO
UseLentiviralExpressionMammalianAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-1
Plasmid#90325PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702DepositorInsertTetR
ExpressionBacterialAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SpyCas9
Plasmid#113034PurposeAAV vector for expression of SpyCas9 in mammalian cellsDepositorInsertSpyCas9
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMrfp
Plasmid#59773PurposeDerived from pCM62; ampR, tetR, PBAD - rfpDepositorInsertrfp
ExpressionBacterialPromoterpBADAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30a-His6-LOXCATmut
Plasmid#140085PurposeA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgTP53(A)
Plasmid#85559PurposeExpresses sgRNA targeting human TP53 around codon F109DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttumor protein p53 (TP53 Human)
ExpressionMammalianAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGT-Kh2
Plasmid#122587PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
10TALE_Pmin_fLuc
Plasmid#160663PurposeExpression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert10TALE_Pmin_fLuc
UseLuciferaseExpressionMammalianPromoterminimalAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197026PurposeVector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized enOsCas12f1
ExpressionMammalianMutationD52R / T132RPromoterCBh, CMV, hU6Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIG-641 JE-TRAC-BCMA(BB)28z-P2A
Plasmid#186127PurposeBCMA-CAR DNA productionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBCMA-CAR (TNFRSF17 Human)
UseCRISPRMutationWTAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-jYCaMP1
Plasmid#135423PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVExpressionMammalianPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Shp2 biosensor
Plasmid#134346PurposeFRET biosensor. To visualize Shp2 activity by FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertShp2 Biosensor
ExpressionMammalianAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mNeonGreen
Plasmid#99132PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreenDepositorInsertDIO-mNeonGreen
UseAAVExpressionMammalianPromoterTREAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3001
Plasmid#91094PurposeModule C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_A1910
Plasmid#91041PurposeModule A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTREX2-P2A-TaCas9
UseCRISPRPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0502
Plasmid#91012PurposeModule A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCsy4-P2A-AtCas9
UseCRISPRPromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO4
Plasmid#24319DepositorInsertAGO4 (AGO4 Human)
ExpressionBacterialAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only