We narrowed to 3,452 results for: ttl

1. pDONR221 CARD8 PAM R394E

   Plasmid

   #169980

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, R394E

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 CARD8 PAM S297A

   Plasmid

   #169976

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, S297A

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 CARD8 FIIND E274R

   Plasmid

   #169967

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   E274R

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 CARD8 FIIND R394E

   Plasmid

   #169971

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   R394E

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR221 CARD8 FIIND F405G

   Plasmid

   #169973

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   F405G

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR221 CARD8 PAM F370G

   Plasmid

   #169979

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, F370G

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221 CARD8 FIIND S297A

   Plasmid

   #169966

   Purpose

   pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   S297A

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221 CARD8 PAM F405G

   Plasmid

   #169981

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, F405G

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221 NLRP1 A59P

   Plasmid

   #166816

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation A59P (Herlin et al., 2020) (no stop codon)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   A59P

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221 Ub-NLRP1 L1215

    Plasmid

    #166828

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221 Ub-NLRP1 S1213

    Plasmid

    #166827

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221 Ub-NLRP1 L1218

    Plasmid

    #166829

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 NLRP1 FIIND (F1079M-L1373)

    Plasmid

    #166831

    Purpose

    Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCK353

    Plasmid

    #129697

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120015170
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK355

    Plasmid

    #129699

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    ilvBN terminator
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOCC176

    Plasmid

    #118891

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV/D377Y-mPCSK9

    Plasmid

    #58376

    Purpose

    Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Has Service

    AAV8

    Insert

    proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    D377Y

    Promoter

    HCRApoE/hAAT

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ269E2

    Plasmid

    #164721

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ262-ABE8e

    Plasmid

    #161523

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    ABE8e-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEVA1

    Plasmid

    #58953

    Purpose

    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.

    Depositor

    Thomas Mason, Thomas Fox

    Article

    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.

    Insert

    VAR1u, VAR1 recoded for expression from the nucleus
    

    Use

    Yeast ecoli shuttle vector

    Tags

    preCox4 mitochondrial matrix targeting signal

    Expression

    Yeast

    Mutation

    Synthetic gene in standard genetic code

    Promoter

    ADH1

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only