We narrowed to 17,082 results for: EMB

1. pAV10.HO5.loxP

   Plasmid

   #63206

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-UBC-XRI-HA

   Plasmid

   #178056

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-HA
   

   Use

   AAV

   Tags

   HA-MBP

   Expression

   Mammalian

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-NOD2-EGFP

   Plasmid

   #131207

   Purpose

   mammalian expression, tet R

   Depositor

   Thomas Kufer

   Article

   Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

   Insert

   NOD2 (NOD2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP VAMP7 (1-220)

   Plasmid

   #42316

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 (1-220) (VAMP7 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRPML1-G-GECO1.2-ERES

   Plasmid

   #207144

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Mcoln1 (MCOLN1 Human)
   

   Tags

   ER-Export Signal (ERES) and G-GECO1.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. W118-1_hZBTB7B

   Plasmid

   #180380

   Purpose

   lentiviral transduction of human ZBTB7B gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-IRF9

   Plasmid

   #71452

   Purpose

   Lentiviral expression of IRF9

   Depositor

   George Stark

   Article

   Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

   Insert

   IRF9 (IRF9 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. ptf-Galectin3

   Plasmid

   #64149

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin3 (LGALS3 Human)
   

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVpuro-PCAF

   Plasmid

   #63705

   Purpose

   retroviral expression of human PCAF in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   PCAF (KAT2B Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   E452G

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

11. METTL7A pEGFP-N2 (890)

    Plasmid

    #62017

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    METTL7A (METTL7A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MBP-FUS_LC_WT

    Plasmid

    #98653

    Purpose

    Expresses MBP-tagged FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-ataxin-3

    Plasmid

    #89975

    Purpose

    Expresses GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TPC2(N257A)-mCherry

    Plasmid

    #135186

    Purpose

    Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of asparagine257 to alanine

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-TEV-CyOFP1

    Plasmid

    #105799

    Purpose

    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-CyOFP1

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3/hArf6(WT)-HA

    Plasmid

    #79424

    Purpose

    Expresses C-terminally HA-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-cFos-XRI-V5

    Plasmid

    #178058

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-V5
    

    Use

    AAV

    Tags

    V5-MBP

    Expression

    Mammalian

    Promoter

    Mouse c-fos promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TPC2(E643A)-mCherry

    Plasmid

    #135187

    Purpose

    Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of glutamate 643 to alanine

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MBP-FUS_FL_12E

    Plasmid

    #98652

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCVpuro-Gcn5

    Plasmid

    #63706

    Purpose

    retroviral expression of mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only