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pSP64T XAR1 DNM1 (DM#102)

Plasmid

#15042

Depositor

Douglas Melton

Article

Hemmati-Brivanlou et al Nature. 1992 Oct 15. 359(6396):609-14.

Insert

Xenopus activin receptor 1 (xstk2 Frog)

Expression

Bacterial

Mutation

Dominant negative mutant

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP64T BMPR (DM#136)

Plasmid

#15068

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pMV2 BMPR (DM#138)

Plasmid

#15069

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a- phage MazG-like/Atd1

Plasmid

#195244

Purpose

Expersses phage MazG-like/Atd1

Depositor

Douglas Feldman

Article

Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

Insert

phage MazG

Tags

Flag and TwinStrep

Expression

Bacterial

Mutation

Catalytic site mutant

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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MetNI (E166Q in MetN)

Plasmid

#78320

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Johnson et al Protein Sci. 2012 Jan;21(1):84-96. doi:

Inserts

MetI
MetN

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

E166Q

Promoter

T7 Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-poly(alanylglycine)

Plasmid

#67003

Purpose

Expresses malE-poly(alanylglycine) in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly(alanylglycine)

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-poly-EAK9

Plasmid

#67010

Purpose

Expresses malE-poly-EAK9 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly-EAK9

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a1-368.E152Q

Plasmid

#17517

Depositor

David McKay

Article

Wang et al Biophys J. 2008 Jan 1. 94(1):L01-3.

Insert

B. subtilis DEAD-box helicase YxiN first two domains

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 369-479; E152Q mutat…

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pSP64T XAR1-myc (DM#121)

Plasmid

#15049

Depositor

Douglas Melton

Article

Hemmati-Brivanlou et al Nature. 1992 Oct 15. 359(6396):609-14.

Insert

Xenopus activin receptor 1 (xstk2 Frog)

Tags

Myc

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pDZ2087

Plasmid

#92414

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

Insert

TEV protease

Tags

MBP (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

tac

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pTXB1-Tn5

Plasmid

#60240

Purpose

IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

Depositor

Rickard Sandberg

Article

Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

Insert

tnpA from pBam1

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7 promoter

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCLIPf-Cox8A Control Plasmid

Plasmid

#101130

Purpose

Expression of CLIP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pTM-NiV-L

Plasmid

#234657

Purpose

Expresses L gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV L gene (L Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-P

Plasmid

#234656

Purpose

Expresses P gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV P gene (Nvgp2 Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-N

Plasmid

#234655

Purpose

Expresses N gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV N gene (N Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSL1180-NiV L(2k)-mCherry/eGFP

Plasmid

#234654

Purpose

Minigenome plasmid of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Inserts

part L gene
mCherry
eGFP

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EEF1A2 in pENTR

Plasmid

#216585

Purpose

gateway cloning donor vector containing EEF1A2

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSNAPf-Cox8A Control Plasmid

Plasmid

#101129

Purpose

Expression of SNAP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Myc tag WT PTB

Plasmid

#23024

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only