We narrowed to 18,385 results for: Met

1. M-SPn-VP64

   Plasmid

   #48674

   Purpose

   Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Mutation

   nuclease-null

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pTE104

   Plasmid

   #59399

   Purpose

   MethyloBrick expression vector, coxB promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PcoxB

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. UMG-SP-1_pET29b

   Plasmid

   #215433

   Purpose

   Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-1
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. UMG-SP-2_pET29b

   Plasmid

   #215434

   Purpose

   Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-2
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ1272

   Plasmid

   #44486

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, MCS
   

   Use

   Multiple cloning site vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAct:dCas9-VP64

   Plasmid

   #78901

   Purpose

   Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGH156_MS2-AID-Hygro

   Plasmid

   #85415

   Purpose

   lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AID and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-C4-mCherry

   Plasmid

   #66530

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter C4

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - C4

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK375 (Hygro-P2A-Stag-3FLAG)

   Plasmid

   #121186

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-Stag-3FLAG
   

   Use

   CRISPR

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TN-XXL pcDNA3

    Plasmid

    #45797

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

    Insert

    TN-XXL
    

    Tags

    ECFP and YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. psiCHECK-2 3xmiR-122T

    Plasmid

    #35672

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    3 miR-122 target sites
    

    Use

    Luciferase

    Promoter

    HSV-TK

    Available Since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREF-GFP kan

    Plasmid

    #122061

    Purpose

    reporter plasmid for termination and terminator override to express GFP. Marked with URA3

    Depositor

    Daniel Reines

    Article

    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

    Inserts

    intergenic terminator from yeast IMD2
    green fluorescent protein
    

    Expression

    Yeast

    Promoter

    GAL1 and GAL1 comes in pYES2

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB2-TagRFP

    Plasmid

    #103997

    Purpose

    Plasmid encoding TagRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CamK2-tTA

    Plasmid

    #210731

    Purpose

    AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

    Depositor

    Wei Xu

    Article

    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    tetracycline-controlled transactivator (tTA)
    

    Use

    AAV

    Promoter

    CamK2

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet-H_peroxisome_C1

    Plasmid

    #85064

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Tags

    mScarlet-H

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U1

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mIFP24-C1

    Plasmid

    #54820

    Purpose

    Localization: C1 Cloning Vector, Excitation: 683, Emission: 704

    Depositor

    Xiaokun Shu, Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Type

    Empty backbone

    Tags

    mIFP24

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. mTagRFP-T-Vinculin-23

    Plasmid

    #58030

    Purpose

    Localization: Focal Adhesions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Vinculin (VCL Human)
    

    Tags

    mTagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pET28a-ybbR-HIS-sfGFP-DocI

    Plasmid

    #58708

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    sfGFP-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Clover-Rab5a-7

    Plasmid

    #56530

    Purpose

    Localization: Ras GTPase, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Rab5a
    

    Tags

    Clover

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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