We narrowed to 29,100 results for: PLE;

1. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEMS1957

   Plasmid

   #82568

   Purpose

   High copy number plasmid containing hs1218 MiniPromoter insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   hshs1218
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pEMS1953

   Plasmid

   #82567

   Purpose

   High copy number plasmid containing hs671 MiniPromoter insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   hs671
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCIG1_UL136_25kDa_Myc

   Plasmid

   #74954

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

   Expression

   Mammalian

   Mutation

   delta 1-77, M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

   Plasmid

   #61796

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Ywhab 3'UTR with miR-200c MRE-1 mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   miR-200c MRE-1 mutated

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

   Plasmid

   #61793

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Xbp1 3'UTR with miR-200c MRE-3 mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   miR-200c MRE-3 mutated

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

    Plasmid

    #61791

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad5 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

    Plasmid

    #61790

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Map3k1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

    Plasmid

    #61789

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    both miR-200c MRE-2 and MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

    Plasmid

    #61786

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Crtap 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.2-R5A-K6A C11orf83-V5

    Plasmid

    #65850

    Purpose

    Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6A

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Mutated UQCC3 (UQCC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R5A-K6A

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.2-R5D-K6D C11orf83-V5

    Plasmid

    #65851

    Purpose

    Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6D

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Mutated UQCC3 (UQCC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R5D-K6D

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

    Plasmid

    #61787

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Fhod1 3'UTR with miR-200c MRE-2 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-2 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

    Plasmid

    #61788

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Tob1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT

    Plasmid

    #61794

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zfp36 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

19. psiCHECK-2-Snail1-3'UTR-MRE-1 MUT

    Plasmid

    #61795

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Snail1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT

    Plasmid

    #61797

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Mapk12 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only