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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

CAMKIIa

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

Plasmid

#202617

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

FRT-JEDI-1P-Kv

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-nirButterfly

Plasmid

#136591

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CMV epArcLight

Plasmid

#85803

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

epArcLight

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; ecliptic pHluorin…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v1

Plasmid

#79537

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 1

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJET1.2-OsMac3

Plasmid

#218542

Purpose

Vector pJET1.2 contains the 158-bp 5′-UTR fragment (from −158 to −1 bp before the ATG) of OsMac3 from Japonica rice genomic DNA.

Depositor

Kirill Demchenko

Article

Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do

Insert

158-bp 5′-UTR fragment (from −158 to −1 bp before the ATG) of OsMac3 from Japonica rice genomic DNA.

Use

Synthetic Biology

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-GPI

Plasmid

#177264

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via CD59 GPI) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

CD59 leader-FRISZ-GPI

Use

AAV

Tags

CD59 GPI and CD59 leader sequence

Promoter

hSyn

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRB133.2

Plasmid

#181950

Purpose

aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)

Tags

mNeonGreen

Expression

Bacterial

Promoter

PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGPTVII-nlsSED1

Plasmid

#178190

Purpose

SED1 biosensor for expression in Arabidopsis thaliana

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

SV40 NLS

Expression

Plant

Promoter

pUBQ10

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HT110_FCMV_QuasAr6b_citrine

Plasmid

#178823

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Lentiviral

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-HisMBP-SED1

Plasmid

#178191

Purpose

SED1 biosensor for expression in Escherichia coli

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

6xHis-MBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHBS1449 SED1-Lenti

Plasmid

#180824

Purpose

SED1 biosensor for stable integration and expression in mammalian cells

Depositor

Rajat Rohatgi

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 osmosensor

Use

Lentiviral

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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HT103_FCMV_QuasAr6a_Citrine

Plasmid

#178822

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6a carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a_Citrine

Use

Lentiviral

Tags

citrine

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

hSyn (human synapsin)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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p1.2-GS

Plasmid

#162770

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Type

Empty backbone

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7

Plasmid

#136466

Purpose

Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO2

Plasmid

#177116

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

GINKO2

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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HA-EglN1-pcDNA3

Plasmid

#18963

Depositor

William Kaelin

Article

Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

Egg laying Nine - 1 (EGLN1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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HT111_Fsyn_FAS(Cre off)_QuasAr6a_Citrine

Plasmid

#178824

Purpose

Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a_citrine

Use

Cre/Lox and Lentiviral

Tags

citrine

Expression

Mammalian

Promoter

hSyn

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine

Plasmid

#178825

Purpose

Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Cre/Lox and Lentiviral

Tags

citrine

Expression

Mammalian

Promoter

hSyn

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+MLS-HyPer7

Plasmid

#136470

Purpose

Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Tandem mitochondrial targeting signal of cytochro…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+IMS-HyPer7

Plasmid

#136469

Purpose

Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

SMAC/DIABLO

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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