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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pCS2 xRor2-Flag

Plasmid

#67164

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

FLag

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pBlueRIP.cOVA

Plasmid

#69595

Purpose

contains cytoplasmic form of ovalbumin (OVA) cDNA in pBlueRIP

Depositor

Francis R. Carbone

Article

Kurts et al J Exp Med. 1996 Sep 1;184(3):923-30.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMT-T-HSV2.3 TCR alpha chain

Plasmid

#74076

Purpose

gBT-I TCR a chain cDNA in pGEM-T

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha clone HSV2.3 (Tcra Mouse)

Expression

Bacterial

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.OVA

Plasmid

#69597

Purpose

contains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)

Depositor

Francis R. Carbone

Article

Blanas et al Science. 1996 Dec 6;274(5293):1707-9.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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SNX19-GFP

Plasmid

#197392

Purpose

Expresses SNX19 in mammalian cells

Depositor

Juan Bonifacino

Article

Saric et al Nat Commun. 2021 Jul 27;12(1):4552. doi:

Insert

SNX19 (SNX19 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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flGalT-SBP-EGFP_puromycin

Plasmid

#65275

Purpose

GalT reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE506

Plasmid

#153279

Purpose

Intermediate sgRNA array cloning vector position 4

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE505

Plasmid

#153278

Purpose

Intermediate sgRNA array cloning vector position 3

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-Gal80

Plasmid

#51620

Purpose

vertebrate expression of full-length Gal80

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pOBCol3.6-MCS

Plasmid

#108311

Purpose

Drive inserted gene in type 1 collagen expressing cells.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 xRor2-EGFP

Plasmid

#67163

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

EGFP

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
flGalT-SBP-mCherry_puromycin

Plasmid

#65276

Purpose

GalT reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pME-basGal80

Plasmid

#51621

Purpose

vertebrate expression of Gal80 preceded by a basal promoter

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Promoter

basal promoter

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
P-Bf-fusA2

Plasmid

#235658

Purpose

Reports Cur activity in Bacteroides fragilis

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides fragilis fusA2 promoter

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-AP-4 β4

Plasmid

#198327

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 β4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Contains L480S substitution

Promoter

ADH1

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-AP-2 σ2

Plasmid

#198345

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-2 σ2

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

R42G substitution, and silent substitutions in co…

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-AP-1 σ1C

Plasmid

#198336

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1C

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

S148C substitution

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-AP-3 σ3A

Plasmid

#198347

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-3 σ3A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-AP-3 σ3B

Plasmid

#198348

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-3 σ3B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-tepsin

Plasmid

#196728

Purpose

Expresses tepsin-GFP in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

tepsin (TEPSIN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Integrase triple controller

Plasmid

#126540

Purpose

Pben-int5; Ptet-bxb1; Pbad-tp901: colE1 Chlo+

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

BenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1

Expression

Bacterial

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK324

Plasmid

#71838

Purpose

Cloned region contains Acetobacter aceti 1023 purE gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK340

Plasmid

#71837

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59N) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK346

Plasmid

#71836

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-EGFP-GPI

Plasmid

#65294

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSDM052

Plasmid

#194341

Purpose

pUC mini-Tn7 vector expressing LseCas13a

Depositor

Joseph Bondy-Denomy

Article

Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

Insert

Cas13a(Lse)

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-Neo

Plasmid

#69123

Purpose

Sleeping Beauty (SB) transposon with G418 resistance

Depositor

Martin Bonamino

Article

Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

Insert

Neo (codon optimized for human)

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

U3MSCV

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH_SBP-EGFP-GPI

Plasmid

#65298

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC026

Plasmid

#227598

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

Depositor

Brittany Adamson

Article

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TurboID-mNeongreen-3xFLAG fusion pos3

Plasmid

#172864

Purpose

C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

Depositor

Mario de Bono

Article

Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

Insert

TurboID

Use

Unspecified

Tags

FLAG, mNeongreen, and myc

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2/1-CAG-Flex-FlpO-WPRE

Plasmid

#171690

Purpose

Cre-dependent expression of Flp-recombinase

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

Insert

Flip-recombinase

Use

AAV

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-shNTC

Plasmid

#217638

Purpose

Dox inducible shRNA non targeting control shRNA

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shNTC

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_flGalT-SBP-mCherry

Plasmid

#65273

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN HAYFP-LOV24

Plasmid

#49570

Purpose

regulate protein stability with light

Depositor

Thomas Wandless

Article

Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

Insert

YFP-LOV24

Use

Retroviral

Tags

HA and YFP

Mutation

delete the last 3 aa from AsLOV2, with I532A muta…

Promoter

LTR

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
ArcLight-A242

Plasmid

#36857

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

semian CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-myrlysin/BORCS5

Plasmid

#194332

Purpose

Expresses myrlysin in mammalian cells

Depositor

Juan Bonifacino

Article

Pu et al Dev Cell. 2015 Apr 20;33(2):176-88. doi:

Insert

BORCS5 (BORCS5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_flGalT-SBP-EGFP

Plasmid

#65272

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE332

Plasmid

#153241

Purpose

M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE333

Plasmid

#153242

Purpose

M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE335

Plasmid

#153244

Purpose

M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHJ8: CadC-VHH-Control

Plasmid

#108243

Purpose

membrane bound transcription activator CadC fusing with anti-RNase A antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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