We narrowed to 13,214 results for: 109

1. pSUM-Kan-MCS2

   Plasmid

   #109289

   Purpose

   Expresses high levels of protein in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pSUM-kan-MCS1-gfp was modified to reorient the Ba…

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a-VHH-tev-std

   Plasmid

   #109423

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. DEP-GFP

   Plasmid

   #109043

   Purpose

   Expression of the Dishevelled DEP domain

   Depositor

   Mariann Bienz

   Article

   Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

   Insert

   dishevelled segment polarity protein 2 (DVL2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Dvl2 DEP domain

   Promoter

   CMV

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 hML23Cm6

   Plasmid

   #109368

   Purpose

   Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   human Melanopsin-mGluR6 chimera (OPN4, GRM6 Human)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. actc1b-lamp1-mKate2

   Plasmid

   #109507

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to lamp1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   lamp1 (lamp1b Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_S100A11_S-100

   Plasmid

   #109804

   Purpose

   Protein expression and purification of S100A11_S-100

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   S100A11_S-100 (S100A11 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

   Plasmid

   #109311

   Purpose

   Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

   Depositor

   William Lagor

   Article

   Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

   Insert

   Ldlr gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3Ai E72A-Cas9n-UGI-NLS

   Plasmid

   #109430

   Purpose

   Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbS8k-RFP_E83K

   Plasmid

   #109412

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   E83K repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_YWHAE_14-3-3

    Plasmid

    #109942

    Purpose

    Protein expression and purification of YWHAE_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAE_14-3-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_GNA11_G-alpha

    Plasmid

    #109911

    Purpose

    Protein expression and purification of GNA11_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA11_G-alpha (GNA11 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB026e

    Plasmid

    #109261

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp_pIII(P29*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codon at position 29 and 184 of gIII

    Promoter

    Ppsp and PproK

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-eArch3.0-mRuby2-ST

    Plasmid

    #109135

    Purpose

    Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eArch3.0-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbS8k-RFP_R46W

    Plasmid

    #109415

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    R46W repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-rab5c-DN

    Plasmid

    #109737

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative rab5c

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab5c (rab5c Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDJSVT89

    Plasmid

    #109303

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR2.1-promoter-mIL-6

    Plasmid

    #109296

    Purpose

    IL-6 promoter reporter construct

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter in pEGFP-N1 replaced with murine IL-…

    Promoter

    murine IL-6 promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. BCL9-BirA*

    Plasmid

    #109147

    Purpose

    To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    BCL9 (BCL9 Human)
    

    Use

    Tags

    BirA(R118G)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-CDC42-DN

    Plasmid

    #109584

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ165

    Plasmid

    #109327

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Promoter EC1.2 enhancer fused to EC1.1 promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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