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pEMG-RIS

Plasmid

#225132

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEMG-AIS

Plasmid

#225133

Purpose

For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pT7RNAP-Q737P

Plasmid

#175356

Purpose

Support plasmid for T7_ori plasmids

Depositor

Sven Panke

Article

Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

Insert

T7RNAP-Q737P

Expression

Bacterial

Mutation

Q737P

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTAL7b

Plasmid

#48706

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pTAL7a

Plasmid

#48705

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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PMT-mApple

Plasmid

#203771

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

PMT-mApple

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSABAD250

Plasmid

#45612

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaBC39

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSADuet760

Plasmid

#45613

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

MjaTFIIB mini intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMKBAD28

Plasmid

#45595

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaEC14

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSABAD332

Plasmid

#45610

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

PhoRadAC46

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSABAD331

Plasmid

#45609

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

TvoVMAC48

Tags

GB1-6

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pJORSF19

Plasmid

#45614

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

PhoRadA precursor

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Article

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSABAD14-172

Plasmid

#61976

Purpose

PhoRadAC6 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadAC6 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-165

Plasmid

#61817

Purpose

PhoRadAdC6 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadAdC6 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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poLuc1

Plasmid

#154128

Purpose

Expresses orthogonal firefly luciferase gene in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

orthogonal firefly luciferase

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pSARSF53-110

Plasmid

#61813

Purpose

TvoVMAN11 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAN11 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSABAD14-98

Plasmid

#61827

Purpose

TvoVMAdN11 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdN11 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRBS wt

Plasmid

#154126

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

wt bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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