We narrowed to 17,071 results for: Por

1. CRISPR-SP-Cas9 reporter

   Plasmid

   #62733

   Purpose

   Mammalian CRISPR-SP-Cas9 reporter

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
   

   Use

   CRISPR and Lentiviral

   Tags

   CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

   Expression

   Mammalian

   Promoter

   EF1-Alpha

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. SRE reporter vector_559

   Plasmid

   #82686

   Purpose

   Cloning vector containing serum response element (SRE) followed by an AscI restriction site

   Depositor

   Harald Janovjak

   Article

   Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE GFP-Grin2a KI

   Plasmid

   #131486

   Purpose

   Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2a Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GFP-Grin2b KI

   Plasmid

   #131487

   Purpose

   Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2b Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP L138 Reporter

   Plasmid

   #119130

   Purpose

   Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   mCherry and eGFP
   

   Use

   Lentiviral

   Mutation

   eGFP L138S

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 7i sgRNA for 4-μHOM reporter

   Plasmid

   #113626

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7i sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

7. betaTrCP mVenus reporter

   Plasmid

   #192458

   Purpose

   Fluorescent reporter for beta-TrCP activity in human cells

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CDC25B (CDC25B Human)
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338

   Promoter

   EF1alpha

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORANGE GFP-Grin1 KI

   Plasmid

   #131485

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE GFP-Camk2a KI

   Plasmid

   #131484

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Camk2a, Camk2a Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE Dlg4-GFP KI

    Plasmid

    #131477

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Dlg4, Dlg4 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p27-mVenus reporter (PB)

    Plasmid

    #190731

    Purpose

    The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    mVenus
    p27
    

    Expression

    Bacterial and Mammalian

    Mutation

    p27-F62A-F64A

    Promoter

    EF1α

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. 7h sgRNA for 4-μHOM reporter

    Plasmid

    #113625

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7h sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pORANGE GFP-Actb KI

    Plasmid

    #131479

    Purpose

    Endogenous tagging of βactin: N-terminal (amino acid position: D2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Actb, Actb Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-SPORT6-hHMGCR1

    Plasmid

    #86085

    Purpose

    expresses the human HMG-CoA reductase isoform 1

    Depositor

    Anne Galy

    Insert

    human HMG-CoA Reductase isoform 1 (HMGCR Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pORANGE GFP-Grin1 KI #3

    Plasmid

    #139659

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV Sport2 mPer2

    Plasmid

    #16204

    Depositor

    Cheng Lee

    Article

    Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

    Insert

    Per2 (Per2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pORANGE GFP-Nlgn3 KI

    Plasmid

    #131501

    Purpose

    Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Nlgn3, Nlgn3 Mouse, Rat)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSV Sport SREBP-1c

    Plasmid

    #8883

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Mol Cell Biol. 1993 Aug . 13(8):4753-9.

    Insert

    SREBP-1c (Srebf1 Mouse)
    

    Expression

    Mammalian

    Mutation

    Active version of SREBP-1c.

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmiR-report-MYB-WT

    Plasmid

    #141114

    Purpose

    Reporter plasmid of WT MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    CMYB (MYB Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pORANGE GFP-Grin1 KI #2

    Plasmid

    #139658

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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