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pEY60

Plasmid

#191057

Purpose

acc-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acc-2 (acc-2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD Delta

Plasmid

#214723

Purpose

Expresses SARS-CoV-2 Delta RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Use

Tags

SpyTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEY39

Plasmid

#191043

Purpose

acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(2.1k) (acr-2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY61

Plasmid

#191058

Purpose

acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(3.7k) (acr-2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-STAG2-wildtype

Plasmid

#31972

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-STAG2 shRNA 3782

Plasmid

#31979

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

STAG2 (STAG2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pLKO-STAG2 shRNA 1221

Plasmid

#31978

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

STAG2 (STAG2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
Lenti_CRISPRi_sgNeg2

Plasmid

#166999

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pαH-S-GSAS-OMICRON.BA.5

Plasmid

#213069

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

S-GSAS-Omicron.BA5 (S )

Use

Tags

HRV 3C cleavage site (before tags) C terminal on …

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pαH-S-RRAR/XBB.1.16

Plasmid

#213037

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

s -RRAR.XBB.1.16 (S )

Use

Tags

HRV 3C cleavage site (before tags) 8X His tag 2X…

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 furin site RRAR …

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pαH-S-GSAS/XBB.1.5

Plasmid

#212991

Purpose

"Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pαH-S-GSAS/XBB.1.5 (S )

Use

Tags

HRV 3C cleavage site (before tags) C terminal on …

Expression

Mammalian

Mutation

(T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pαH-S-GSAS/XBB.1.16

Plasmid

#212992

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pαH-S-GSAS/XBB.1.16 (S )

Use

Tags

HRV 3C cleavage site (before tags) C terminal on …

Expression

Mammalian

Mutation

(T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q, E180…

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyTag-RBD Omicron BQ.1.1

Plasmid

#214725

Purpose

Expresses SARS-CoV-2 Omicron BQ.1.1 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Use

Tags

SpyTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyTag-RBD Omicron XBB.1.5

Plasmid

#214724

Purpose

Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Use

Tags

SpyTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RISeGFP (CC#353)

Plasmid

#16131

Depositor

Connie Cepko

Article

Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Insert

eGFP

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC mPlum IX

Plasmid

#37355

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

mPlum

Use

Tags

Expression

Mutation

Promoter

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC TdTomato IX

Plasmid

#37348

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

Tdtomato

Use

Tags

Expression

Mutation

Promoter

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-STAG2-A223Stop

Plasmid

#31973

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Alanine 223 to Stop codon

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only