We narrowed to 1,318 results for: V2

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pNN_v2

Plasmid

#32182

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer NN

Expression

Bacterial

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lentiCRISPR v2 sgKEAP1-2

Plasmid

#186459

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Publication

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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STING-V2

Plasmid

#124264

Purpose

C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Tags

Venus2

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2-HygR-EGFP

Plasmid

#167188

Purpose

Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

Depositor

Yin Shen

Publication

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Hygromycin B phosphotransferase and EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTALEN_v2 (NN)

Plasmid

#32191

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Nuclease, NN for 0.5 repeat

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHD_v2

Plasmid

#32183

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer HD

Expression

Bacterial

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNG_v2

Plasmid

#32181

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer NG

Expression

Bacterial

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNI_v2

Plasmid

#32180

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer NI

Expression

Bacterial

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Publication

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MIGR1-U6gRNA1-Filler-v1

Plasmid

#237399

Purpose

For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v2.

Depositor

Zuoming Sun

Publication

Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6, PGK

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

Plasmid

#133793

Purpose

U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Publication

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

CjeCas9 sgRNA entry vector

Expression

Mammalian

Mutation

V2-TGAA linker sgRNA architecture from Kim et al.…

Promoter

U6

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp102-v2 scaffold

Plasmid

#157978

Purpose

pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Publication

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CROP-seq-mTurquoise2-v2-no-scaffold

Plasmid

#228522

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

Depositor

Jellert Gaublomme

Publication

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Insert

mTurquoise2

Use

Lentiviral

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CROPseq-multi-Puro (pRW1441)

Plasmid

#216217

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

Depositor

Paul Blainey

Publication

Walton et al bioRxiv [Preprint]. 2024 Mar 17:2024.03.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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LentiGuide-puro-NLS-GFP

Plasmid

#185473

Purpose

Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Publication

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LentiCRISPRv2-ATG5

Plasmid

#99573

Purpose

Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

Depositor

Edward Campbell

Publication

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting ATG5 (ATG5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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px459 VQR

Plasmid

#101715

Purpose

sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

Depositor

Andrew Holland

Publication

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q and T1337R)

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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