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We narrowed to 5,067 results for: codon optimized

Showing: 281 - 300 of 5067 results

  1. pGEX4T1-Opt WWP1

    Plasmid
    #179327
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1
    Depositor
    Arthur Haas
    Insert
    WWP1 (WWP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Available since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179327-plasmid-map-sequence-id-358706

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  2. pGEX4T1-WWP1F792D

    Plasmid
    #179328
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1 F792D
    Depositor
    Arthur Haas
    Insert
    WWP1 F792D (WWP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    F792D mutation; Codon Optimized for expression in…
    Promoter
    Available since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179328-plasmid-map-sequence-id-358707

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  4. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Use
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87854-plasmid-map-sequence-id-175127

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  6. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison
    Article
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Expression
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    45945-plasmid-map-sequence-id-116251

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  7. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47754-plasmid-map-sequence-id-68222

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  8. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61736-plasmid-map-sequence-id-256988

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  10. pCS2+/N-Halo

    Plasmid
    #184417
    Purpose
    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    Halo-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184417-plasmid-map-sequence-id-362159

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  12. pCS2+/C-Halo

    Plasmid
    #184418
    Purpose
    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    Halo-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184418-plasmid-map-sequence-id-373502

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  13. pJY3948

    Plasmid
    #113338
    Purpose
    6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression
    Depositor
    Suzanne Sandmeyer
    Article
    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.
    Insert
    2-pyrone synthase, codon optimized for Y. lipolytica
    Use
    Tags
    6XHis
    Expression
    Yeast
    Mutation
    Please see depositor comments
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113338-plasmid-map-sequence-id-218737

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  14. pNB784

    Plasmid
    #60163
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Use
    Tags
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60163-plasmid-map-sequence-id-96372

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  15. pNB788

    Plasmid
    #60165
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Use
    Tags
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60165-plasmid-map-sequence-id-96374

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  16. pDL0_212

    Plasmid
    #210778
    Purpose
    C2_Pyrearinus termitilluminans click beetle luciferase ('Eluc' version - human-codon optimized)
    Depositor
    Dmitri Nusinow
    Article
    Lee et al bioRxiv 2025.06.20.660755
    Insert
    Pyrearinus termitilluminans beetle luciferase ('Eluc' version - human-codon optimized)
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    210778-plasmid-map-sequence-id-421287

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  20. pJM108

    Plasmid
    #134160
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    KMLL, E. coli codon optimized, CRISPR/Cas9 harden…
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134160-plasmid-map-sequence-id-271380

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Showing: 281 - 300 of 5067 results