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pGEM-T Dmel Or45b

Plasmid

#59656

Purpose

Probe for Drosophila melanogaster Or45b expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or45b (Or45b Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or49a

Plasmid

#59661

Purpose

Probe for Drosophila melanogaster Or49a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or49a (Or49a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or49b

Plasmid

#59662

Purpose

Probe for Drosophila melanogaster Or49b expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or49b (Or49b Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or59a

Plasmid

#59664

Purpose

Probe for Drosophila melanogaster Or59a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or59a (Or59a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or63a

Plasmid

#59667

Purpose

Probe for Drosophila melanogaster Or63a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or63a (Or63a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or65a

Plasmid

#59668

Purpose

Probe for Drosophila melanogaster Or65a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or65a (Or65a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or65b

Plasmid

#59669

Purpose

Probe for Drosophila melanogaster Or65b expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or65b (Or65b Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or67a

Plasmid

#59671

Purpose

Probe for Drosophila melanogaster Or67a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or67a (Or67a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or7a

Plasmid

#59636

Purpose

Probe for Drosophila melanogaster Or7a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or7a (Or7a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-7Zf-wild

Plasmid

#23160

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Ribonuclease assay sub1

Use

In vitro transcription

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pGEM-7Zf-deleted

Plasmid

#23161

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Ribonuclease assay sub2

Use

In vitro transcription

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pGEM cLrdr (CT#900)

Plasmid

#13950

Depositor

Cliff Tabin

Article

Essner et al Nature. 2002 Jul 4. 418(6893):37-8.

Insert

Lrdr

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Expression

Mammalian

Promoter

Ef1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-miRFP-MAP4-MTBD

Plasmid

#171487

Purpose

T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

miRFP

Mutation

Aminoacids 658-1126

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-QF2

Plasmid

#122563

Purpose

Integration of QF2 sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-QF2-SV40pA

Use

Cloning

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-mito-GEM-GECO1

Plasmid

#32461

Purpose

Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCashGem-Xyl2-URA3-LINEAR

Plasmid

#174840

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

ENO1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

Plasmid

#217662

Purpose

expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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