Addgene

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mApple-TNFa-RUSH

Plasmid

#166902

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA3(GAT)

Plasmid

#79436

Purpose

Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA3 (GGA3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 107-286

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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Man2a1-myc

Plasmid

#163648

Purpose

Expresses myc tagged Man2a1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 2A member 1 (MAN2A1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Silent mutation was made to introduce a XbaI rest…

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hArfaptin1

Plasmid

#206404

Purpose

Mammalian expression of hArfaptin1

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin1 (ARFIP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-GGA1

Plasmid

#178459

Purpose

Expresses GGA1 in mammalian cells

Depositor

Juan Bonifacino

Article

Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. d

Insert

GGA1 (GGA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Cerulean-GalT

Plasmid

#11930

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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Sar1b-mOrange2

Plasmid

#166899

Purpose

mammalian expression of Sar1b tagged with mOrange2

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mOrange2

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hArfaptin2

Plasmid

#206405

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEF6 VPS51-V5-6His

Plasmid

#175502

Purpose

V5 tagged VPS51 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

Insert

VPS51 (VPS51 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

A661V- please see depositor comments

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA1(GAT)

Plasmid

#79435

Purpose

Expresses GST-tagged GAT domain of GGA1 (141-327 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA1 (GGA1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 141-327

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG/hArf4(WT)-mCherry

Plasmid

#79406

Purpose

Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pDDGFP-Leu2d-GgKDELR2

Plasmid

#123618

Purpose

Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-tev-APEX2

Plasmid

#117751

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

Depositor

Martin Spiess

Article

Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

Insert

anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope

Use

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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hsp70:Stathmin1-mKate2

Plasmid

#105969

Purpose

heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Stathmin1 (STMN1 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-OSBP

Plasmid

#32495

Depositor

Alex Toker

Article

Nhek et al Mol Biol Cell. 2010 Jul 1;21(13):2327-37

Insert

OSBP (OSBP Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(T31N)-HA

Plasmid

#79405

Purpose

Expresses C-terminally HA-tagged Arf4(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T31N

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(Q71L)-HA

Plasmid

#79404

Purpose

Expresses C-terminally HA-tagged Arf4(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Gln 71 Leu

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-HA

Plasmid

#79403

Purpose

Expresses C-terminally HA-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-CERT PH domain

Plasmid

#58725

Purpose

Expresses GFP-Tagged CERT PH domain in yeast

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-EGFP

Plasmid

#79407

Purpose

Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only