We narrowed to 6,070 results for: nls

1. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

   Plasmid

   #218710

   Purpose

   AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

   Plasmid

   #170182

   Purpose

   Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   mCherry
   

   Use

   Tags

   3XFlag and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

   Plasmid

   #176538

   Purpose

   Mammalian expression of nuclear localized AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5

   Plasmid

   #161555

   Purpose

   Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

   Plasmid

   #170183

   Purpose

   Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   sgBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1

   Plasmid

   #237890

   Purpose

   Cre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting Cre

   Depositor

   Rui Lin

   Insert

   sgRNA-Cre
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

   Plasmid

   #237891

   Purpose

   Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre

   Depositor

   Rui Lin

   Insert

   sgRNA-Cre
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3

   Plasmid

   #237892

   Purpose

   Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre

   Depositor

   Rui Lin

   Insert

   sgRNA-Cre
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

   Plasmid

   #203219

   Purpose

   Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCasMINI-V4-QNZF-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM573 ZF6x6-C 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

    Plasmid

    #161560

    Purpose

    Inducible expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the ZF6x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    ZF6x6-C

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJM576 ZF2x6-C 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

    Plasmid

    #161562

    Purpose

    Inducible expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the ZF2x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-intC-ZF1-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    ZF2x6-C

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM524 EF1α 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

    Plasmid

    #161553

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-intC-ZF1-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

    Plasmid

    #161554

    Purpose

    Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61591

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205319

    Purpose

    All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISTfu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190034

    Purpose

    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A134V,A140V,A141V,A143V

    Promoter

    CBh, SV40, hU6

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61593

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    HA, NLS, and OLLAS tag

    Expression

    Mammalian

    Mutation

    K175R and K736R (deUb mutations)

    Promoter

    Available since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205316

    Purpose

    All-in-one AAV vector expressing ISAam1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAam1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

    Plasmid

    #205317

    Purpose

    All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISYmu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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