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We narrowed to 4,673 results for: pcr

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Showing: 281 - 300 of 4673 results

  2. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  4. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    163095-plasmid-map-sequence-id-320590

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  7. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Publication
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189620-plasmid-map-sequence-id-379033

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  9. pCR-5'Myt1outSB605

    Plasmid
    #22738
    Depositor
    Lynn Hudson
    Insert
    Myelin Transcription Factor 1 (Myt1 Mouse)
    Use
    Pcr cloning vector
    Available since
    Dec. 18, 2009
    Availability
    Academic Institutions and Nonprofits only
    22738-plasmid-map-sequence-id-10401

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  12. pAAV-Ef1a-DIO-PPO-Venus

    Plasmid
    #139505
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Publication
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has service
    AAV8 and AAV9
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Promoter
    Ef1a
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    139505-plasmid-map-sequence-id-276175

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  13. pMaCTag-Z13

    Plasmid
    #120056
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    Pcr template
    Tags
    mCherry
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120056-plasmid-map-sequence-id-232665

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  14. pMaCTag-Z05

    Plasmid
    #120048
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Use
    Pcr template
    Tags
    eGFP
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120048-plasmid-map-sequence-id-232654

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Showing: 281 - 300 of 4673 results