We narrowed to 784 results for: transfection

1. pmCherry_sgRNA-ver2

   Plasmid

   #126776

   Purpose

   gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   sgRNA (for SpCas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFP Y93H CC editing reporter

   Plasmid

   #198885

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Y93H, Gly92(GGT-to-GGC)

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. eGFP Y93H AC editing reporter

   Plasmid

   #198883

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Y93H, Gly92(GGT-to-GGA)

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmCherry_gRNA

   Plasmid

   #80457

   Purpose

   gRNA expression vector with mCherry transfection control

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Inserts

   sp Cas9 gRNA
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin promoter

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PAGFP_NS2B3

   Plasmid

   #195755

   Purpose

   Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

   Depositor

   Partha Mondal

   Article

   Varghese et al bioRxiv 2022.12.18.520514

   Insert

   Dengue (Type-2) NS2B3
   

   Use

   Tags

   PA-GFP

   Expression

   Mammalian

   Mutation

   No mutations

   Promoter

   CMV

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

   Plasmid

   #194925

   Purpose

   mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

   Depositor

   Hao Xu

   Article

   Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCaRNA1&2

   Plasmid

   #86087

   Purpose

   Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA1, RNA2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBMN-dGBP1-TagBFP

   Plasmid

   #80087

   Purpose

   MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   dGBP1-TagBFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

   Plasmid

   #184394

   Purpose

   Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eGFP Y93H GC editing reporter

    Plasmid

    #198884

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGG)

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p-715MRPprom-MRPcucuc

    Plasmid

    #87199

    Purpose

    Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    RMRP (RMRP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GAGAG91-95 to CUCUC

    Promoter

    human RMRP

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPWCh

    Plasmid

    #124238

    Purpose

    Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

    Depositor

    Peter Vilmos

    Article

    Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UASp

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBOB-3xFlag-hGpr75

    Plasmid

    #236746

    Purpose

    Express 3xFlag N-terminal tagged wild-type human GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    GPR75 (GPR75 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EF1a-mGL-bGH

    Plasmid

    #235299

    Purpose

    mGreenLantern expression to serve as a transfection marker, EF1a-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EFS-mGL-bGH

    Plasmid

    #235255

    Purpose

    mGreenLantern expression to serve as a transfection marker, EFS-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS (human EF1a short, intronless)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBOB-3xFlag-mGpr75

    Plasmid

    #236744

    Purpose

    Express 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    Gpr75 (Gpr75 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBOB-3xFlag-mGpr75-L144P

    Plasmid

    #236745

    Purpose

    Express 3xFlag N-terminal tagged mouse GPR75-L144P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    Gpr75 (Gpr75 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    L144P

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a-tagBFP-bGH

    Plasmid

    #235257

    Purpose

    tagBFP expression to serve as a "filler" plasmid for transfections

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to remove BsaI sites in tagBFP

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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