We narrowed to 704 results for: transfection

1. pAC1821_pCR8-SMN2-DN-RG6-SA

   Plasmid

   #118655

   Purpose

   Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   SMN2-DN-RG6-SA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFP Y93H GC editing reporter

   Plasmid

   #198884

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Y93H, Gly92(GGT-to-GGG)

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-715MRPprom-MRPcucuc

   Plasmid

   #87199

   Purpose

   Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   RMRP (RMRP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GAGAG91-95 to CUCUC

   Promoter

   human RMRP

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xG1 PylT sfGFP 150TAG

   Plasmid

   #154767

   Purpose

   plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPWCh

   Plasmid

   #124238

   Purpose

   Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

   Depositor

   Peter Vilmos

   Article

   Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UASp

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-TaGu-ENT-3xF-ORF

   Plasmid

   #238488

   Purpose

   For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   R1109->A

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-mGL-bGH

   Plasmid

   #235299

   Purpose

   mGreenLantern expression to serve as a transfection marker, EF1a-driven

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mGreenLantern
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a (human EF1a)

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. EFS-mGL-bGH

   Plasmid

   #235255

   Purpose

   mGreenLantern expression to serve as a transfection marker, EFS-driven

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mGreenLantern
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS (human EF1a short, intronless)

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBOB-3xFlag-mGpr75

   Plasmid

   #236744

   Purpose

   Express 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

   Depositor

   Zhao Zhang

   Article

   Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

   Insert

   Gpr75 (Gpr75 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBOB-3xFlag-mGpr75-L144P

    Plasmid

    #236745

    Purpose

    Express 3xFlag N-terminal tagged mouse GPR75-L144P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    Gpr75 (Gpr75 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    L144P

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a-tagBFP-bGH

    Plasmid

    #235257

    Purpose

    tagBFP expression to serve as a "filler" plasmid for transfections

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to remove BsaI sites in tagBFP

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-TaGu-WT-3xF-ORF

    Plasmid

    #238487

    Purpose

    For production of C-term flag-tagged TaGu R2 ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-TaGu-RTD-3xF-ORF

    Plasmid

    #238490

    Purpose

    For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    DD660/661->AA

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-TaGu-END-3xF-ORF

    Plasmid

    #238489

    Purpose

    For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    DD1057/1070->AA

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGEN_GFP

    Plasmid

    #235611

    Purpose

    Encodes GFP. For use in transfections as a negative control without epitope tags.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus-Bik-del72-136-pEGFP-C3

    Plasmid

    #191411

    Purpose

    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).

    Depositor

    David Andrews

    Insert

    Bcl-2-interacting killer (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    with deletion of amino acids 72-136 (del72-136) i…

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-GgVCL/*TBM

    Plasmid

    #162790

    Purpose

    MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

    Depositor

    Laura Lafon-Hughes

    Article

    et al PeerJ

    Insert

    Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
    

    Use

    Tags

    IRES-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    changed Gly 454 to Val

    Promoter

    beta actin

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-GFP

    Plasmid

    #137763

    Purpose

    Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Flag-NPM1wt

    Plasmid

    #131810

    Purpose

    Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RDX-mCherry

    Plasmid

    #214476

    Purpose

    Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    RDX (RDX Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Sequence matches cDNA clone MGC:48283 IMAGE:52844…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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