We narrowed to 15,088 results for: pET

1. pET-51b(+)_NAD-Snifit

   Plasmid

   #113658

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a_3xFlag-nsp14 (SARS-CoV-2)

   Plasmid

   #169159

   Purpose

   To express SARS-CoV-2 nsp14 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
   

   Tags

   3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-29b(+)_NSP4ss_SerM_NucA-myc

   Plasmid

   #218191

   Purpose

   Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Tags

   NSP4 signal secretion peptide and myc

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-51b(+)_NADP-Snifit

   Plasmid

   #113913

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a T7pCONS TIR-1 sfGFP

   Plasmid

   #154463

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Tags

   His-thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-MCN-10His-StayGold

   Plasmid

   #211360

   Purpose

   Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   StayGold
   

   Tags

   10xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-Nsp14-TEV-Strep

   Plasmid

   #154953

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET-28a(+)-FGF2-K128N

   Plasmid

   #120284

   Purpose

   Produce human recombinant FGF2 K128N in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 2 (FGF2 Human, Synthetic)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His6-SUMO-MproC145A

   Plasmid

   #172653

   Purpose

   Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

   Depositor

   Stephen Harrison

   Article

   MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

   Insert

   SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHis Tag and SUMO

   Expression

   Bacterial

   Mutation

   Catalytic Cysteine 145 mutated to Alanine

   Promoter

   T7

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-45b-mCherry-NPM1

    Plasmid

    #194546

    Purpose

    Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    NPM1 (Npm1 Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-6His-MBP-MpAgo

    Plasmid

    #79249

    Purpose

    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

    Depositor

    Jennifer Doudna

    Article

    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

    Insert

    Marinitoga piezophila Argonaute
    

    Use

    CRISPR

    Tags

    6XHis-tag + MBP + PreScission Cleavage Site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-G6-vioABE-4A6-vioC

    Plasmid

    #73439

    Purpose

    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    PG6 and P4A6 (orthogonal T7-lac variants)

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-22b DT 51E/148K

    Plasmid

    #11081

    Depositor

    John Collier

    Insert

    DT
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    51E/148K, catalytically inactive

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-TS2126 RnlA-Strep

    Plasmid

    #159350

    Purpose

    Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

    Depositor

    Takashi Ito

    Article

    Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

    Insert

    TS2126 RNA ligase
    

    Tags

    His6 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Tags

    no

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-26b-Nb127D01-HA-His

    Plasmid

    #171566

    Purpose

    Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-HA-His (CXCR2 Human)
    

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-45b-mEGFP-HMGB1-WT

    Plasmid

    #194543

    Purpose

    Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28C-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204402

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-SENP1 (catalytic domain)

    Plasmid

    #16356

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP1 catalytic domain (SENP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 419 to 644 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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