We narrowed to 17,391 results for: puro

1. pLenti spCas9 T2A TagRFP-T P2A puro

   Plasmid

   #122200

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9
   TagRFP-T
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

   Plasmid

   #194925

   Purpose

   mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

   Depositor

   Hao Xu

   Article

   Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)

   Plasmid

   #208395

   Purpose

   To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   Human MRE11 (MRE11 Human)
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   DB1delta/DB2delta

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

   Plasmid

   #134869

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome b5 type A (CYB5A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-98

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

   Plasmid

   #200637

   Purpose

   Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   Vif
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T27stop

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB-CMV-MCS-Puro GEM-LTB4

   Plasmid

   #202641

   Purpose

   Expresses the LTB4 sensor GEM-LTB4 in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   GEM-LTB4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121185

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Tet-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   OsTIR1 codon optimized for human cells

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

   Plasmid

   #162117

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to ProtC-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEpicVector3mC_Puro-T2A-mCherry-VSVg-FLAG-AU1

   Plasmid

   #162108

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

    Plasmid

    #162084

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-FLAG

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

    Plasmid

    #162091

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. stblHIF: pLenti-PGK-stblHIF-PGK-Puro

    Plasmid

    #177202

    Purpose

    encoding stblHIF under PGK promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    stblHIF
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P402A/P577G/N813A

    Promoter

    PGK

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHage2-EF1a-TERT K78E-IRES-Puro

    Plasmid

    #182591

    Purpose

    expresses TERT K78E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K78E

    Promoter

    EF-1a

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV Puro DEST (w118-1) eGFP

    Plasmid

    #107505

    Purpose

    Expresses eGFP, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid

    #100596

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

    Plasmid

    #120562

    Purpose

    Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRL-gRNA(anti-cGAS)-Cas9-Puro

    Plasmid

    #130908

    Purpose

    gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB-CMV-MCS-Puro GEM-LTB4mut

    Plasmid

    #202642

    Purpose

    Expresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cells

    Depositor

    Balázs Enyedi

    Article

    Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

    Insert

    GEM-LTB4mut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

    Plasmid

    #117405

    Purpose

    SpyCas9 sgRNA 1 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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