We narrowed to 5,181 results for: PID;

1. pMK282 (mCherry2-Bsr)

   Plasmid

   #72798

   Purpose

   C-terminal tagging with mCherry2 using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mCherry2-Bsr
   

   Use

   CRISPR

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaM-1

   Plasmid

   #172767

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-1
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPI227

   Plasmid

   #113231

   Purpose

   safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Safe haven knock-in plasmid for the act5 locus,li…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   act5

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX303 GFP-PIK3C2A

   Plasmid

   #162000

   Purpose

   Lentiviral expression vector containing PIK3C2A tagged with GFP

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LZRS-RasG12V

   Plasmid

   #21203

   Depositor

   Paul Khavari

   Article

   Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

   Insert

   Ras (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pQCi-mB2m-sgRNA

   Plasmid

   #154090

   Purpose

   pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting murine B2M locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH-nuc-roGFP2-GPXC71S

   Plasmid

   #191760

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-GPXC71S
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   GPX has no peroxidatic Cysteine 71, which is repl…

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaG-29

   Plasmid

   #172753

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-29
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hyper7 in pT3TS-Dest

   Plasmid

   #194296

   Purpose

   In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   Hyper7
   

   Use

   In vitro transcription of mrna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGK:TRL-EFS:iRFP670

    Plasmid

    #68475

    Purpose

    Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    iRFP670
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and pGK

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACE-GIRK4-S

    Plasmid

    #172425

    Purpose

    Expression of full-length, human GIRK4 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TB:7xSMAD_RE:MiniP

    Plasmid

    #133879

    Purpose

    7 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies the SMAD DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

    Plasmid

    #199352

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-3C

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199364

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lyn-CFP-GAI(1-92)

    Plasmid

    #37311

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-GFAP-JEDI-1P-WPRE

    Plasmid

    #202616

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. J23103_GB

    Plasmid

    #65991

    Purpose

    MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Constitutive promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. J23107_GB

    Plasmid

    #65999

    Purpose

    MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Constitutive promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge