We narrowed to 4,893 results for: PIR

1. p53-(1-393)-E285K-N268D

   Plasmid

   #35089

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Contains aa 1-393 with N269D and E285K mutations

   Promoter

   T7

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVRa16_3622

   Plasmid

   #49670

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF16_3622
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7749 Bicistronic_ires_hygro

   Plasmid

   #64374

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain

   Plasmid

   #183397

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of human caspase-4 fused to catalytic domain of canine caspase-1/4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dCpf1-BE0

   Plasmid

   #107678

   Purpose

   Expresses dLbCpf1-BE0 in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D832A/E925/D1148A

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_SERF2_HUMAN (pBS0751)

   Plasmid

   #185239

   Purpose

   For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   SERF2_HUMAN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PKM-IRES-hrGFP

   Plasmid

   #73405

   Purpose

   Mammalian expression of mouse PKM and hrGFP

   Depositor

   Raymond Swanson

   Article

   Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

   Insert

   PKM (Prkcz Mouse)
   

   Use

   Tags

   3xFLAG and pIRES-hrGFP-2a

   Expression

   Mammalian

   Mutation

   truncated, constitutively active form

   Promoter

   CMV

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

8. LN-entry

   Plasmid

   #128012

   Purpose

   Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag
   

   Use

   Lamdan entry vector

   Tags

   LN-3xFlag

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST_MAHS_RAMVA (pBS0310)

   Plasmid

   #185169

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SARS-CoV-2 SL1 alone-Luciferase

    Plasmid

    #191482

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

    Insert

    SARS-CoV-2 5' UTR SL1 alone
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Contains only the stem-loop1 region of SARS-CoV-2…

    Promoter

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVRa12_807

    Plasmid

    #49663

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF12_807
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pST_DHN_AtCor47 (pBS0784)

    Plasmid

    #185254

    Purpose

    For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHN_AtCor47
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV.U6.gLacZ.Cas9-T2A-GFP

    Plasmid

    #170361

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-TetO-Txnip

    Plasmid

    #178437

    Purpose

    doxycycline-inducible expression of Mouse Txnip in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Txnip (Txnip Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.U6gT28.13.EFS-NS.H2B-RFP

    Plasmid

    #170388

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS-NS

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVRa15_436

    Plasmid

    #49667

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF15_436
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRa28_1088

    Plasmid

    #49683

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF28_1088
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-p160(1-200)

    Plasmid

    #43

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pVRa11_987

    Plasmid

    #49661

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF11_987
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHelA12

    Plasmid

    #176493

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

    Depositor

    Jian-Dong Huang, Xiaodong Cheng

    Article

    Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

    Insert

    SARS-CoV NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    V570I

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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