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pCAG-p65AD-GBP6

Plasmid

#49440

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Article

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
YFC43

Plasmid

#89488

Purpose

Gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFP

Depositor

Alejandro Ferrando

Article

Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

Type

Empty backbone

Tags

YFP-C (aa 154-240)

Expression

Plant

Promoter

2x35S

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-SOX2

Plasmid

#47540

Purpose

Tet inducible knockdown of SOX2.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

SOX2 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEF-I-GFP GX

Plasmid

#45443

Depositor

John Brigande

Article

Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

Type

Empty backbone

Tags

IRES EGFP

Expression

Mammalian

Promoter

human EF1α

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-mGLIS1

Plasmid

#33273

Purpose

Retroviral expression of mouse Glis1

Depositor

Shinya Yamanaka

Article

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1, Gateway casette A (Glis1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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WNK2 WT

Plasmid

#24569

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2 (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pCAM-kan-R4R3

Plasmid

#71275

Purpose

Binary destination vector for plant transformation. Kanamycin resistance.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Type

Empty backbone

Use

Gateway Cloning

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4-AARE-luc2P-Hygro

Plasmid

#101787

Purpose

Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

3xAARE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-YFP

Plasmid

#67146

Purpose

Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-YFP (ADRB2 Human, Aequorea victoria)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-Bcl2-Cb5

Plasmid

#18004

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

ER targeted Bcl-2 (BCL2 Human)

Tags

Flag

Expression

Mammalian

Mutation

C terminal of Bcl-2 replaced with Cytochrome b5. …

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
pMM2-hPMCA1b

Plasmid

#47758

Purpose

mammalian expression of human PMCA1b

Depositor

John Penniston, Emanuel Strehler

Article

Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

Insert

PMCA1 (ATP2B1 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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EF.deltaBHES1.Ubc.GFP

Plasmid

#24982

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

DNA-binding domain disrupted by E43A, K44A, R47A …

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
Krox20 promoter pGL3-TATA

Plasmid

#21260

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

Krox20 promoter (Egr2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAd-Oct4

Plasmid

#19768

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

Insert

Oct4 (Pou5f1 Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT miniAID-EGFP

Plasmid

#101713

Purpose

For amplification of a miniAID-EGFP for C-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

miniAID-EGFP

Tags

miniAID-EGFP

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGF1-4eCOL2A1

Plasmid

#97210

Purpose

Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

Depositor

Ryan Porter

Article

De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

Insert

4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

COL2A1 regulatory elements

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCX-DI-2A

Plasmid

#74671

Purpose

Mammalian expression of DI-2A (hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

Inserts

CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTfR-PATagRFP

Plasmid

#31946

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

TfR

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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ER-mCherry-eMagA

Plasmid

#162248

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-mCherry-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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