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Showing: 3001 - 3020 of 18102 results

  1. mTagRFP-T2-Farnesyl-5

    Plasmid
    #58037
    Purpose
    Localization: Plasma Membrane, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pETDest

    Plasmid
    #124245
    Purpose
    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124245-plasmid-map-sequence-id-257540

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  5. pHR-pSFFV-TAF15N-mCherry-sspB

    Plasmid
    #122436
    Purpose
    Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.
    Depositor
    Clifford Brangwynne
    Article
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    TAF15 (TAF15 Human)
    Use
    Lentiviral
    Tags
    mCherry and sspB
    Expression
    Mammalian
    Mutation
    Contains only amino acids 1-208
    Promoter
    SFFV
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    122436-plasmid-map-sequence-id-243727

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  6. AICSDP-27:SLC25A17-mEGFP

    Plasmid
    #101784
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17
    Depositor
    Allen Institute for Cell Science
    Insert
    SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101784-plasmid-map-sequence-id-197677

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  7. C1QBP

    Plasmid
    #32863
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C1QBP (C1QBP Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acids E89-Q282 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pcDNA3.1-ccdB-V5

    Plasmid
    #214985
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    V5
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214985-plasmid-map-sequence-id-440020

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  9. pENN.AAV.TBG.PI.ffLuciferase.RBG

    Plasmid
    #105538
    Purpose
    AAV expression of firefly luciferase from TBG promoter
    Depositor
    James M. Wilson
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Expression
    Mammalian
    Promoter
    TBG
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105538-plasmid-map-sequence-id-202429

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  10. pGL3 Enhancer Vector

    Plasmid
    #212938
    Purpose
    Firefly luciferase vector for investigating regions controlling transcription
    Depositor
    Debrya Groskreutz
    Type
    Empty backbone
    Use
    Luciferase
    Mutation
    WT
    Available Since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212938-plasmid-map-sequence-id-419193

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  11. pJAT7

    Plasmid
    #204292
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204292-plasmid-map-sequence-id-405844

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  12. AICSDP-80:UBTF-HaloTag

    Plasmid
    #133963
    Purpose
    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF
    Depositor
    Allen Institute for Cell Science
    Insert
    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    Use
    CRISPR
    Tags
    HaloTag-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    133963-plasmid-map-sequence-id-264347

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  13. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. FCbAIGW

    Plasmid
    #26669
    Depositor
    Gerardo Morfini
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    IRES-EGFP
    Expression
    Mammalian
    Available Since
    June 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    26669-plasmid-map-sequence-id-12617

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  17. pGL4.82(hRluc/Puro)

    Plasmid
    #212933
    Purpose
    Renilla luciferase vector for investigating regions controlling transcription
    Depositor
    Pete Stecha
    Type
    Empty backbone
    Use
    Luciferase
    Mutation
    WT
    Available Since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212933-plasmid-map-sequence-id-419194

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  20. pB6-Hipp11-DTA-Capecchi-for

    Plasmid
    #126649
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Available Since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126649-plasmid-map-sequence-id-247127

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Showing: 3001 - 3020 of 18102 results