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pSCrhaB2plus

Plasmid

#164226

Purpose

Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

Depositor

Silvia Cardona

Publication

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Expression

Bacterial

Promoter

Rhamnose-inducible

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-HA-LMW-FGF2

Plasmid

#157663

Purpose

Expresses FGF2 in mammalian cell

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deleted amino acid 1-134

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

Plasmid

#127392

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

Depositor

Ryohei Yasuda

Publication

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Aspartic acid and Threon…

Promoter

pCAG

Available since

July 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z07

Plasmid

#124790

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P22

Plasmid

#120033

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Publication

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pUB-cSypHer

Plasmid

#84733

Purpose

Expresses SypHer in plant cells-biosensor for pH

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

SypHer

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Publication

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-q-pep

Plasmid

#47437

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Publication

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pFRT/TO/HIS/FLAG/HA-BTF3

Plasmid

#38075

Depositor

Markus Landthaler

Publication

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

BTF3 basic transcription factor 3 (BTF3 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.Tau2N4R

Plasmid

#226396

Purpose

Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Publication

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C

Expression

Bacterial

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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APEX2-V5-G3BP1_pTRE-G418

Plasmid

#202013

Purpose

expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Publication

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

APEX2-V5-G3BP1

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE-Tight

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-TdT

Plasmid

#126450

Purpose

Expresses human TdT in mammalian cells. (pTBL206)

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL

Plasmid

#111397

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON hM4Di-mCherry (Gi-coupled DREADD for neuronal silencing), W3SL cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Publication

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
hM4Di-mCherry

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Promoter

hSynapsin

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTMB304_ZFcharm_Kv1_Prnp_SCR

Plasmid

#220842

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB301_ZFcharm_Kv1_Prnp_SPM

Plasmid

#220840

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCHFLAGGR

Plasmid

#1279

Depositor

Susan Lindquist

Publication

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GR (Nr3c1 Rat)

Tags

FLAG

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pEC3135 (pERASE)

Plasmid

#220306

Purpose

Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

Depositor

Emmanuelle Charpentier

Publication

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Synthetic Biology

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Publication

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Expression

Bacterial

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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