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pEM.H02L

Plasmid

#198667

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI2_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H01L

Plasmid

#198665

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

X3_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8

Plasmid

#175796

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_SS

Plasmid

#175798

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples'

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_SS

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_SH2sub6

Plasmid

#175799

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_SH2sub6

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP9sub3

Plasmid

#175794

Purpose

de novo thick circular tandem repeat protein trimer formed from 3-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP9sub3

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub6

Plasmid

#175795

Purpose

de novo thick circular tandem repeat protein tetramer formed from 6-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub6

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

Plasmid

#163925

Purpose

Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor

Use

Cre/Lox

Tags

tagBFP

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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D63_Ld_100680

Plasmid

#135876

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

flagellar glycoprotein-like protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D57_Ld_190640

Plasmid

#135870

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

extracellular receptor, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS Exon

Plasmid

#110119

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati Exons 1-3

Plasmid

#110113

Purpose

Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 deltaC 350 (stop)

Plasmid

#67612

Purpose

bacterial expression of GST-PRMT9 missing residues 351-845

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

Stop codons placed at aa 351 and 352 to create C …

Promoter

tac

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
HOXB4-FLAG-pSP65

Plasmid

#20990

Depositor

Corey Largman

Article

Chang et al Genes Dev. 1995 Mar 15. 9(6):663-74.

Insert

HOXB4 (HOXB4 Human)

Use

In vitro trans/trans

Mutation

Missing first 14 amino acids

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
HOXB13 (human) Myc-tag-pSP72

Plasmid

#8682

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Use

Tnt in vitro

Tags

Myc

Expression

Bacterial

Mutation

new NsiI site at ATG start

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-PKAsub-YPet-Kras

Plasmid

#138206

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub-YPet-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-PKCsub-RLuc8N-NES

Plasmid

#138213

Purpose

Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

mCherry-PKCsub-RLuc8N-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Tags

Sapphire

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MCP-Rep-X-RT∆R

Plasmid

#239384

Purpose

For circular RNA-mediated inverse prime editor using MCP-Rep-X-M MLV-RT∆RNase H in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

MCP, Rep-X, M-MLV-RT(∆RNase H)

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

∆RNase H in M-MLV RT

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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