We narrowed to 5,882 results for: pcDNA 3.1

1. pcDNA3-mPC1_4119-eGFP-3HA

   Plasmid

   #108393

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-WT-WLS-HA-IRES-GFP

   Plasmid

   #178061

   Purpose

   Expression of WLS-HA protein (WT)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACE2-WT(732)-IgG1

   Plasmid

   #154104

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-hPC1_4106-eGFP-3HA

   Plasmid

   #108399

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-sACE2-T92Q-Fc(IgG1)

   Plasmid

   #145170

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-Flag-SPRTN-Y117C

   Plasmid

   #110216

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y117C, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-sACE2-T92Q-sfGFP

   Plasmid

   #145178

   Purpose

   Mammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Linker GSGGSGSGG and superfolder GFP

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-sACE2-T92Q-Fc(IgA1)

   Plasmid

   #145162

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

   Plasmid

   #108404

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-mPC1_4148-eGFP-3HA

    Plasmid

    #108396

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    variant ID rs13468401 missense variant R4237Q

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 TEV-GFP-FLAG LIC cloning vector (6LD)

    Plasmid

    #166835

    Purpose

    Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sACE2v2.4(732)-IgG1

    Plasmid

    #154106

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-sACE2v2(732)-IgG1

    Plasmid

    #154105

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Xiap-Myc K322/328R

    Plasmid

    #11834

    Depositor

    Guy Salvesen

    Insert

    XIAP K322/328R (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K322/328R

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

15. PME-1 S156A (inakt.mut) pcDNA-StrepIII

    Plasmid

    #119293

    Purpose

    Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center

    Depositor

    Jukka Westermarck

    Insert

    PPME1 (PPME1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S156A

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid

    #155313

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid

    #155009

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-LoxP-mKate-LoxP-EGFP

    Plasmid

    #167940

    Purpose

    CMV promoter-LoxP-mKate-LoxP-EGFP

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    LoxP-mKate-LoxP-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-Flag-SPRTN-E112A

    Plasmid

    #110215

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge