We narrowed to 6,969 results for: tet on
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Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3ā²-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pS5
Plasmid#231614PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signalsDepositorInsertPbad-TetR-sfGFP-Ptet-AraC-mKate2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbad/PtetAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pS4
Plasmid#231613PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signalsDepositorInsertPtet-AraC-mKate2-Pbad-TetR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbad/PtetAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMT-flp
Plasmid#67274PurposeConjugally transferable Flp recombinase plasmid for unmarked mutagenesisDepositorInserttetA, flp, repA101, oriR101
UseFlp recombinase plasmid, temperature sensitiveTagsHis-SUMO (flp)ExpressionMutationPromotertetA promoter of plasmid pACYC184 originAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTS2163
Plasmid#169632PurposeTier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)DepositorInsertOTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
UseTagsExpressionMammalianMutationPromotertetO7-PhCMVmin / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRAN334
Plasmid#120814PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)DepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTc-4xDQNAT
Plasmid#128393PurposeIn vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin fragment C domain
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Q22YU3-KO
Plasmid#170313PurposePlasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selectionDepositorInsertQ22YU3 knockout
UseTetrahymena somatic gene knockout vectorTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Q22MS1-KO
Plasmid#170312PurposePlasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selectionDepositorInsertQ22MS1 knockout
UseTetrahymena somatic gene knockout vectorTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IRES-Hygro
Plasmid#102423PurposeMammalian expression vector for tetracycline-transactivator (rtTA), IRES-HygroDepositorInserttetracyclin-transactivator (rtTA)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTG06-Amp-FRT
Plasmid#128275PurposeDeg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD184
Plasmid#90953PurposeExpresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutivelyDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23114, PltetO-1, and PphsAAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQdC12a
Plasmid#191356PurposeClostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expressionDepositorInsertsPGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR
UseTagsExpressionBacterialMutationPromoterPGus2A-TetO2/1 and miniPthlAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-PURO
Plasmid#58246PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selectionDepositorInsertPuro(R)
UseLentiviral and RNAi; Gateway destionation vectorTagsTetR-NLS-T2AExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pANTO5
Plasmid#131106PurposeTo generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integraseDepositorInserttetR gene by pFRA61 (Boldrin et al 2010)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTlight-4xDQNAT
Plasmid#128394PurposeIn vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin light chain domain
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating E234A mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGN33
Plasmid#131105PurposeAn integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutantsDepositorInsertP-furA102tetO-pip
UseTagsExpressionBacterialMutationthe furA102 promoter was mutated with the insertiā¦PromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-Flag-H3.3
Plasmid#47980Purposetetracycline-inducible expression of human Flag tagged H3F3ADepositorInsertH3F3A (H3-3A Human)
UseTagsFlagExpressionMammalianMutation288 bp T to C synonymous mutationPromoterCMV promoter with tetracycline operatorAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only