We narrowed to 30,664 results for: PLE

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pEE024

Plasmid

#176781

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

301

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE025

Plasmid

#176782

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

302

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE026

Plasmid

#176783

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

303

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE027

Plasmid

#176784

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

304

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE028

Plasmid

#176785

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

305

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE029

Plasmid

#176786

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

306

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE030

Plasmid

#176787

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

307

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE006

Plasmid

#176763

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

102

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE007

Plasmid

#176764

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

103

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE008

Plasmid

#176765

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

104

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE009

Plasmid

#176766

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

201

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE010

Plasmid

#176767

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

202

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG13 (1-197)

Plasmid

#188074

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Expression

Mammalian

Mutation

codon-optimized ATG13 (1-197)

Promoter

CMV

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP*

Plasmid

#175238

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-MCS-LexA-VP16-MiniWhite

Plasmid

#165900

Purpose

Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPK-352

Plasmid

#157922

Purpose

pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

Depositor

Phillip Kyriakakis

Article

Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

Inserts

tPcyA
IRES
HO1
FD
FNR

Use

Synthetic Biology; Optogenetics

Tags

Mitochondrial Targeting Sequence, Mitochondrial T…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

Plasmid

#124068

Purpose

CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)

Depositor

Gregor Bucher

Article

He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1

Insert

eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40

Use

CRISPR and Cre/Lox

Expression

Insect

Promoter

bhsp68

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1942

Plasmid

#105867

Purpose

High copy number plasmid containing Ple265 (PCP2 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple265

Promoter

Ple265 (PCP2 MiniPromoter)

Available Since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Opto-GPR148

Plasmid

#106048

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR148; Opto-GPR148; E2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR148 (GPR148 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR176

Plasmid

#106060

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR176 (GPR176 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR183

Plasmid

#106062

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR183; Opto-GPR183; F4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR183 (GPR183 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR78

Plasmid

#106033

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR78; Opto-GPR78; C11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR78 (GPR78 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR141

Plasmid

#106045

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR141; Opto-GPR141; D11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR141 (GPR141 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR62

Plasmid

#106028

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR62; Opto-GPR62; C6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR62 (GPR62 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR32

Plasmid

#106015

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR32; Opto-GPR32; B5)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR32 (GPR32 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR33

Plasmid

#106016

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR33; Opto-GPR33; B6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR33 (GPR33 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104619

Purpose

Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10

Tags

FLAG

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-AtSDT (JBEI-11535)

Plasmid

#87934

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSDT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

SDT (SDT Mustard Weed)
4CL5 (4CL5 Mustard Weed)

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1207

Plasmid

#29104

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple235

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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GPR97-DuET

Plasmid

#213294

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPR97 (ADGRG3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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