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We narrowed to 9,474 results for: BLI;

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Showing: 6041 - 6080 of 9474 results

  1. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51095
    Purpose
    Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    ChIEF(E162A/T198C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E162A/T198C
    Promoter
    EF1a
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51095-plasmid-map-sequence-id-79317

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  2. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  4. EP300

    Plasmid
    #39018
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    EP300 (EP300 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  7. BRDT

    Plasmid
    #38898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRDT (BRDT Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

    Plasmid
    #185696
    Purpose
    AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter
    Depositor
    Lynette Lim
    Insert
    mScarlet, T2A, Synaptophysin-EGFP
    Use
    AAV and Cre/Lox
    Tags
    mScarlet palmitoylation, Synaptophysin fused to E…
    Expression
    Mammalian
    Promoter
    mDlx
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185696-plasmid-map-sequence-id-366332

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  9. CAMK2D

    Plasmid
    #38987
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2D (CAMK2D Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pSB700-mouse-ACTC1-Puro

    Plasmid
    #128326
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128326-plasmid-map-sequence-id-255029

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  11. FH

    Plasmid
    #38978
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FH (FH Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

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  13. pdCas9-tdTomato-VPR

    Plasmid
    #232632
    Purpose
    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato-VPR
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232632-plasmid-map-sequence-id-462149

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  14. pTXB1-FIS

    Plasmid
    #51564
    Purpose
    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    FIS
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51564-plasmid-map-sequence-id-78815

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  15. GAK

    Plasmid
    #39042
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GAK (GAK Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. TH mAb_BCN53.1.1D10_HC_Mouse IgG2a

    Plasmid
    #182896
    Purpose
    Mammalian expression of TH mouse mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-TH (human) heavy chain (TH Mouse)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182896-plasmid-map-sequence-id-360557

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  17. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid
    #140026
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140026-plasmid-map-sequence-id-275838

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  18. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  19. pLEX307-ACE2-G418

    Plasmid
    #158450
    Purpose
    Constitutive lentiviral expression of human ACE2.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158450-plasmid-map-sequence-id-308176

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  20. pGreenII Tfs-494::LUC

    Plasmid
    #44467
    Purpose
    Expresses a luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation
    Depositor
    Roger Hellens, Ross Johnson
    Insert
    A luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation
    Use
    Luciferase; Plant transformation
    Mutation
    A disruption was made between the first and secon…
    Promoter
    35S Promoter
    Available Since
    April 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    44467-plasmid-map-sequence-id-472941

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  21. pGEX6P1-DEST-FLAG

    Plasmid
    #119754
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    FLAG and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119754-plasmid-map-sequence-id-234028

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  22. CD55-full length

    Plasmid
    #220874
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 (CD55 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMW
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220874-plasmid-map-sequence-id-443039

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  23. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

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  24. pFRT-TODestGFP_HuC

    Plasmid
    #65762
    Purpose
    expresses GFP-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available Since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65762-plasmid-map-sequence-id-111834

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  25. p2Bac.His.XPB

    Plasmid
    #39216
    Depositor
    Aziz Sancar
    Insert
    XPB (ERCC3 Human)
    Tags
    His
    Expression
    Insect
    Promoter
    Pph
    Available Since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  26. pNY-352

    Plasmid
    #231049
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to guinea pig Fc
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Available Since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231049-plasmid-map-sequence-id-457169

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  27. p2Bac.His.XPD

    Plasmid
    #39217
    Depositor
    Aziz Sancar
    Insert
    XPD (ERCC2 Human)
    Tags
    His (HHYHHH)
    Expression
    Insect
    Mutation
    XPD starts at aa4
    Promoter
    Pph
    Available Since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  28. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

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  29. Cin85-3SH3-pCMV6-AN-GFP

    Plasmid
    #51137
    Purpose
    Express turbo GFP tagged three SH3 domains of Cin85 (truncation) in mammalian cells
    Depositor
    Agnieszka Swiatecka-Urban
    Insert
    Cin85 (SH3KBP1 Human)
    Tags
    TurboGFP
    Expression
    Mammalian
    Mutation
    contains 3 SH3 domains
    Promoter
    CMV
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  31. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  32. CECR2

    Plasmid
    #39066
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CECR2 (CECR2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. KIF3A406

    Plasmid
    #129768
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    129768-plasmid-map-sequence-id-271499

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  34. GLRX5

    Plasmid
    #39007
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX5 (GLRX5 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. BRD1

    Plasmid
    #39095
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD1 (BRD1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. pKG96

    Plasmid
    #63072
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-A (CENPA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    A12V, C75Y, and L121F mutations compared to GenBa…
    Available Since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63072-plasmid-map-sequence-id-134723

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  37. MAT1A

    Plasmid
    #38852
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAT1A (Mat1a Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pNY-345

    Plasmid
    #231045
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to rabbit Fc
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Available Since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231045-plasmid-map-sequence-id-457177

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  40. pNY-347

    Plasmid
    #231047
    Purpose
    Used for GFP staining
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to mouse Fc
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Available Since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231047-plasmid-map-sequence-id-457176

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Showing: 6041 - 6080 of 9474 results