We narrowed to 10,133 results for: ada

1. pAAV-CAG-miRFP680-P2A-GFP

   Plasmid

   #197164

   Purpose

   Expresses the protein of miRFP680-P2A-GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP680-P2A-GFP
   

   Use

   AAV

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2369 - intron GG1 - 250bp

   Plasmid

   #159877

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG1 - 250bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-EZH2

   Plasmid

   #222528

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   EZH2 (EZH2 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-LOV-VP16

   Plasmid

   #42499

   Purpose

   LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   FKF1 (N-LOV, G128D mutant) fused with VP16
   

   Tags

   F-type VP16 activation domain and HA tag

   Expression

   Mammalian

   Mutation

   G128D 1-168aa

   Promoter

   CMV

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

   Plasmid

   #191140

   Purpose

   Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

   Plasmid

   #159583

   Purpose

   Luciferase reporter for ribosome pausing

   Depositor

   Shintaro Iwasaki

   Article

   Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

   Inserts

   XBP1u pause site
   EIF2S3 5UTR
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-YTHDC2

   Plasmid

   #222558

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   YTHDC2 (YTHDC2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC25

   Plasmid

   #177319

   Purpose

   Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

   Depositor

   Rafal Tomecki

   Article

   Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

   Insert

   TtCutA 240-603
   

   Tags

   6xHis-SUMOTag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-NELFE

   Plasmid

   #222559

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of NELFE

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   NELFE (NELFE Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

    Plasmid

    #191146

    Purpose

    Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    Bdnf.t1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Yellow Cameleon-Nano65/pcDNA3

    Plasmid

    #51965

    Purpose

    mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano65
    

    Expression

    Mammalian

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pPB EF1a CCR6-eLOV-TEVcs-Gal4

    Plasmid

    #173119

    Purpose

    Expresses CCR6 eLOV receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCR6-eLOV-TEVcs-Gal4
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xmNeonGreen_UtrCH

    Plasmid

    #129607

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Utrophin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

    Plasmid

    #191170

    Purpose

    EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (plant)
    

    Expression

    Plant

    Mutation

    WT

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY1461_SERPINA1_CCA32_5avidity Cre

    Plasmid

    #193193

    Purpose

    Express Cre conditional upon RNA transcript

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    Cre
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-MBP-huErCas12a

    Plasmid

    #174685

    Purpose

    Expresses 6xHis-MBP tagged ErCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huErCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis

    Expression

    Bacterial

    Mutation

    Q926R mutation is observed as indicated from the …

    Promoter

    T7

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 V5 hHR23A

    Plasmid

    #13053

    Depositor

    Steven Grossman

    Article

    Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

    Insert

    hHR23A (RAD23A Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    R325K and L355P relative to NP_001257291.1

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. EcNusGFL

    Plasmid

    #113122

    Purpose

    Expresses full-length E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-cT3G-cERP2-DDX4

    Plasmid

    #222527

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of DDX4

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    DDX4 (DDX4 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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