We narrowed to 31,863 results for: ica
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Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0757
Plasmid#133509PurposeExpression of 3GFP tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRU5HT1-inLuc
Plasmid#58954PurposeHTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.DepositorInsertinLuc
UseRetroviralPromoterCMV/R/U5Available SinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionMutationCodon-optimized for N. crassaAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-TEX264-FLAG
Plasmid#128258PurposeExpresses TEX264 in mammalian cellsDepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MmXYRS-4xM15
Plasmid#155343PurposePlasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.DepositorInsertHumanized MmXYRS/tRNAM15
ExpressionMammalianPromoterCMV/U6Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-Z3EV
Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(1-93).ER.VP16
Plasmid#108216PurposeYeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domainDepositorInsertGal4 DBD-ERalpha HBD-VP16
ExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH1Available SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2high-mCherry
Plasmid#104063PurposeExpresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherryDepositorInsertCRY2high-mCherry
ExpressionMammalianMutationCRY2PHR E490RPromoterCMVAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-FLAG-FAM134Bs
Plasmid#128261PurposeExpresses FAM134Bs in mammalian cellsDepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
Tags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pADH118
Plasmid#90983PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionPromoterT7 for each geneAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-tdTomato
Plasmid#104067PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomatoDepositorInsertCRY2low-tdTomato
ExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlanineAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC64422
Plasmid#191768PurposeCRISPR mutagenesis in Medicago truncatulaDepositorInsertSpCas9
UseCRISPRExpressionPlantAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB4906
Plasmid#106150PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
optoAKT
Plasmid#207164PurposeLight-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathwayDepositorInsertCRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
ExpressionMammalianAvailable SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
FEOX-PBCS-TBF2- MC2
Plasmid#234406PurposeTool for genetically encoded fluorescent biosensor of cellular iron microenvironmentDepositorInsertsFEOX1 CONTROL
FEOX
UseSynthetic BiologyTagsmTagBFP2ExpressionMammalianPromoterEF1-alpha and hpgkAvailable SinceOct. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-mCherry
Plasmid#104065PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherryDepositorInsertCRY2low-mCherry
ExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only